ATM and CHK-1 activity in young versus senescent human fibroblasts
| dc.contributor.author | Axelrad, Jessica D., author | |
| dc.contributor.author | Ray, Andrew F., author | |
| dc.date.accessioned | 2007-01-03T05:50:57Z | |
| dc.date.available | 2007-01-03T05:50:57Z | |
| dc.date.issued | 2006 | |
| dc.description.abstract | ATM and CHK1 are two different DNA repair proteins. As such, they are an important factor in preventing tumors caused by multiple damages and subsequent mutations in the DNA strands. As people get older they have an increased chance of getting cancer because their cells have had more opportunities to mutate. We hypothesized that it is also possible a person's chance of getting cancer increases with age because the DNA repair proteins, such as ATM and CHK1, become less efficient with age. Protein activity was evaluated by first damaging cellular DNA in young, middle aged, and senescent human fibroblasts, by exposing them to gamma radiation. A Western blot analysis was then performed to determine the concentration of phosphorylated (or activated) ATM and CHK1 proteins in these different aged cells. The data showed that the ATM proteins become more phosphorylated the more radiation the cells are exposed to (as expected), but that, especially in the 10-G cells, the young and middle aged cells have a higher concentration of activated ATM than the old cells. There is a similar pattern with the CHK1 protein blot. The activated protein concentration increases with an increased dose of radiation, but while the 10-G young and middle aged cells have a significant CHK1 concentration, the near senescent cells do not. This decrease in DNA repair protein activation contributes to a person's increased likelihood of cancer. Not only have the older cells undergone more opportunities for a genetic mutation to occur, but the ability of the cell to repair DNA damage appears to be compromised. Future studies will include DNA repair assays. | |
| dc.description.award | College Honors. | |
| dc.format.medium | Student works | |
| dc.format.medium | posters | |
| dc.identifier.uri | http://hdl.handle.net/10217/542 | |
| dc.language | English | |
| dc.language.iso | eng | |
| dc.publisher | Colorado State University. Libraries | |
| dc.relation.ispartof | 2006 Projects | |
| dc.rights | Copyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright. | |
| dc.subject | DNA repair | |
| dc.subject | Fibroblasts | |
| dc.subject | Cancer | |
| dc.title | ATM and CHK-1 activity in young versus senescent human fibroblasts | |
| dc.type | StillImage | |
| dc.type | Text | |
| dcterms.rights.dpla | This Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). | |
| thesis.degree.discipline | Veterinary Medicine and Biomedical Sciences | |
| thesis.degree.discipline | Environmental and Radiological Health Sciences |
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