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Extraction and charaterization of humus with reference to clay-associated humus

dc.contributor.authorAnderson, D. W., author
dc.contributor.authorPaul, E. A., author
dc.contributor.authorSt. Arnaud, R. J., author
dc.contributor.authorAgricultural Institute of Canada, publisher
dc.date.accessioned2007-01-03T06:06:39Z
dc.date.available2007-01-03T06:06:39Z
dc.date.issued1974-08
dc.description.abstractAn extraction-fractionation method was developed with which it is possible to isolate 60–67% of the humus of Chernozemic and Luvisolic soils. Two humic acid fractions were obtained: (1) a conventional alkali-pyrophosphate extractable HA-A; (2) a clay-associated HA-B fraction isolated after ultrasonic dispersion, in water, of the residue of the alkali-pyrophosphate extraction. As compared to the HA-A, the HA-B fractions had lower C contents but greater N contents, narrower C:H ratios, higher molecular weights, and less resistance to hydrolysis in 6 N HCl. It was concluded that the HA-B is a weakly humified, potentially labile humic constituent stabilized by adsorption to clay. Spectral measurements on low-ash (< 2 %) humic acids showed a positive correlation between extinction coefficient at 280 nm (E280) and C:H ratio, and negative correlations between E280 and percent of hydrolyzable C and N. The E4:E6 ratio was related to molecular weight, as determined by agar gel filtration, increasing with decrease in molecular weight.
dc.format.mediumborn digital
dc.format.mediumarticles
dc.identifier.bibliographicCitationAnderson, D. W., E. A. Paul, and R. J. St. Arnaud, Extraction and Charaterization of Humus with Reference to Clay-Associated Humus. Canadian Journal of Soil Science 54, no. 3 (August 1974): 317-323. https://dx.doi.org/10.4141/cjss74-041.
dc.identifier.doihttps://dx.doi.org/10.4141/cjss74-041
dc.identifier.urihttp://hdl.handle.net/10217/80856
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado State University. Libraries
dc.relation.ispartofFaculty Publications
dc.rights©1974 Agricultural Institute of Canada.
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.
dc.subjectorganic heteropolymers
dc.subjectsoil organic matter
dc.subjectextraction-fractionation method
dc.subjecthumus
dc.titleExtraction and charaterization of humus with reference to clay-associated humus
dc.typeText

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Extraction and charaterization of humus with reference to clay-associated humus