Repository logo
 

Understanding the roles of TAL effectors in Xanthomonas oryzae interactions with rice

Simple item page
dc.contributor.authorCorral, Rene, author
dc.contributor.authorLeach, Jan E., advisor
dc.contributor.authorVerdier, Valérie, advisor
dc.contributor.authorHess, Ann, committee member
dc.date.accessioned2007-01-03T06:10:15Z
dc.date.available2015-01-31T05:57:00Z
dc.date.issued2013
dc.description.abstractTranscription activator like (TAL) effector proteins are virulence factors that are secreted by Xanthomonas oryzae. Some TAL effectors contribute to virulence by activating transcription of plant host susceptibility genes thereby modulating the plant's physiology and creating a more pathogen favorable environment. Some TAL effectors activate transcription of disease resistance genes. Because most X. oryzae strains encode many (between 8 and 26) genes for TAL effectors, it is difficult to evaluate the function of individual TAL in the plant-pathogen interaction. In this study, we introduced the use of a TAL deficient strain of X. oryzae that allows study of individual TAL effectors. We demonstrated that the TAL deficient strain could deliver TAL to rice, and that subtle differences in TAL virulence functions could be measured in interactions with rice when delivered by this strain. Plants have evolved resistance genes that detect or recognize TAL effectors and activate resistance responses. Using the TAL deficient X. oryzae delivery system, we isolated a TAL that activates resistance in plants which are homozygous for the recessive resistance gene xa5. The TAL, Avrxa5P86, is predicted to target many transcription factors. Avrxa5P86 not only interacted with xa5 to confer resistance, but also exhibited a novel resistance interaction with Azucena and Nipponbare, both homozygous for the Xa5 allele. Our discovery of Avrxa5P86 is of presents a novel interaction that raises new evolutionary questions about TAL effectors and/or resistance genes in rice.
dc.format.mediumborn digital
dc.format.mediummasters theses
dc.identifierCorral_colostate_0053N_12155.pdf
dc.identifierETDF2013500365BSPM
dc.identifier.urihttp://hdl.handle.net/10217/81009
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado State University. Libraries
dc.relation.ispartof2000-2019
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.
dc.titleUnderstanding the roles of TAL effectors in Xanthomonas oryzae interactions with rice
dc.typeText
dcterms.embargo.expires2015-01-31
dcterms.embargo.terms2015-01-31
dcterms.rights.dplaThis Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
thesis.degree.disciplineBioagricultural Sciences and Pest Management
thesis.degree.grantorColorado State University
thesis.degree.levelMasters
thesis.degree.nameMaster of Science (M.S.)

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Corral_colostate_0053N_12155.pdf
Size:
5.02 MB
Format:
Adobe Portable Document Format
Description:
Download

Collections

2000-2019
Theses and Dissertations