Evaluation of clinical parameters for detection of early endpoint criteria in guinea pigs experimentally infected with Mycobacterium tuberculosis
| dc.contributor.author | Williams, Wendy R., author | |
| dc.contributor.author | Izzo, Angelo, advisor | |
| dc.contributor.author | Kendall, Lon, committee member | |
| dc.contributor.author | Johnston, Matt, committee member | |
| dc.date.accessioned | 2015-08-28T14:35:05Z | |
| dc.date.available | 2017-08-14T06:30:24Z | |
| dc.date.issued | 2015 | |
| dc.description.abstract | Tuberculosis (TB) is a global epidemic caused by the bacteria Mycobacterium tuberculosis. Despite extensive research and funding to improve diagnostic and treatment strategies, the emergence of multi drug resistant and extensively drug resistant strains of M. tuberculosis are on the rise. Vaccines present a solution to the failing diagnostic and treatment strategies by preventing incidence of disease. Guinea pigs are a common animal model to test TB vaccine candidates, but are difficult to evaluate in terms of overall health status. We hypothesized that using parameters within the complete blood count, serum biochemistry, and urinalysis assays we would identify biomarkers that could be used as early endpoint criteria for guinea pigs experimentally infected with M. tuberculosis. Using groups of BCG-vaccinated or saline-treated guinea pigs we measured biomarkers over the life of guinea pigs both pre-challenge and after challenge with laboratory strain M. tuberculosis H37Rv. Our study showed significant differences between groups of guinea pigs, as well as compared to pre-challenge values. Specifically, blood urea nitrogen, urine specific gravity, serum proteins, alanine transferase, hematocrit, leukocytes, and body weight should be monitored when establishing early endpoint criteria in guinea pigs experimentally infected with M. tuberculosis. | |
| dc.format.medium | born digital | |
| dc.format.medium | masters theses | |
| dc.identifier.uri | http://hdl.handle.net/10217/167089 | |
| dc.language | English | |
| dc.language.iso | eng | |
| dc.publisher | Colorado State University. Libraries | |
| dc.relation.ispartof | 2000-2019 | |
| dc.rights | Copyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright. | |
| dc.title | Evaluation of clinical parameters for detection of early endpoint criteria in guinea pigs experimentally infected with Mycobacterium tuberculosis | |
| dc.type | Text | |
| dcterms.embargo.expires | 8/14/2017 | |
| dcterms.embargo.terms | 8/14/2017 | |
| dcterms.rights.dpla | This Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). | |
| thesis.degree.discipline | Microbiology, Immunology, and Pathology | |
| thesis.degree.grantor | Colorado State University | |
| thesis.degree.level | Masters | |
| thesis.degree.name | Master of Science (M.S.) |
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