Repository logo
 

A derivatization protocol for mycolic acids detection using liquid chromatography/mass spectrometry

Simple item page
dc.contributor.authorZurita Urrea, Paulina, author
dc.contributor.authorBelisle, John T., advisor
dc.contributor.authorJones, Robert, committee member
dc.contributor.authorSalman, Mo, committee member
dc.date.accessioned2007-01-03T08:40:50Z
dc.date.available2007-01-03T08:40:50Z
dc.date.issued2012
dc.description.abstractNew tools for the diagnosis and control of Tuberculosis are major challenges. In this context the use of biomarkers can be applied for detecting characteristic signatures from the tuberculosis-infected host and the pathogen. Mycolic acids are considered as a hallmark of the Mycobacterium genus being abundant in the mycobacterial cell wall. In this study a derivatization protocol was tested to enhance the detection of mycolic acid after the attachment of a quaternary amine and analysis of the derivatized products in the positive ionization mode with liquid chromatography/mass spectrometry. Three groups were considered i) mycolic acid standard ii) human urine spiked with mycolic acid standard, and iii) human serum spiked with mycolic acid standard. Each group included the analysis of a set of non-derivatized mycolic acids in positive and negative ionization mode, and derivatized mycolic acids in positive mode. The derivatization process applied to the mycolic acid standard and to the urine samples spiked with mycolic did not improve the ion volume value compared to the respective non-derivatized samples. Serum samples, however, showed a significant enhancement in the ion volume of the different mycolic acids analyzed compared to the non-derivatized serum samples (α=0.05). The method detection limit for the three groups was also achieved. Urine and serum samples spiked with mycolic acids showed higher detection limits compared to the mycolic acid standard; this was expected due the lipid extraction protocol and the complex nature of these fluids. The derivatization protocol did not improve the method detection limit compared to the non-derivatized samples. The overall results make the derivatization protocol questionable to be applied routinely in biological samples. However, the results obtained after the derivatization of serum samples could point to the advantages of using a derivatization protocol to study possible interactions between mycolic acids and other molecules present in serum that could be impeding their detection.
dc.format.mediumborn digital
dc.format.mediummasters theses
dc.identifierZurita_colostate_0053N_11421.pdf
dc.identifierETDF2012400466MIPA
dc.identifier.urihttp://hdl.handle.net/10217/75304
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado State University. Libraries
dc.relation.ispartof2000-2019
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.
dc.subjectbiomarker
dc.subjectderivatization
dc.subjectmass spectrometry
dc.subjectmycolic acids
dc.titleA derivatization protocol for mycolic acids detection using liquid chromatography/mass spectrometry
dc.typeText
dcterms.rights.dplaThis Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
thesis.degree.disciplineMicrobiology, Immunology, and Pathology
thesis.degree.grantorColorado State University
thesis.degree.levelMasters
thesis.degree.nameMaster of Science (M.S.)

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Zurita_colostate_0053N_11421.pdf
Size:
3.44 MB
Format:
Adobe Portable Document Format
Description:
Download

Collections

2000-2019
Theses and Dissertations