Skn-1, Nrf homolog, mediates cannabidiol cellular stress responsive effects in Caenorhabditis elegans
| dc.contributor.author | Alsulami, Abdullatif M., author | |
| dc.contributor.author | Moreno, Julie, advisor | |
| dc.contributor.author | McGrath, Stephanie, committee member | |
| dc.contributor.author | LaRocca, Tom, committee member | |
| dc.contributor.author | Arnold, Olivia, committee member | |
| dc.date.accessioned | 2023-06-01T17:27:12Z | |
| dc.date.available | 2023-06-01T17:27:12Z | |
| dc.date.issued | 2023 | |
| dc.description.abstract | Alzheimer's disease (AD) is a neurodegenerative disease that is affecting an increasing number of the aged population worldwide. AD is characterized by the accumulation of amyloid beta (Aβ) and tau hyperphosphorylation along with a failure in redox homeostasis. The hallmarks of neurodegenerative diseases include the increased generation of reactive oxygen species (ROS) which is tightly controlled by an antioxidant defense mechanism under physiological conditions. This research aimed to utilize various strains of the model organism C. elegans to understand the mechanism of cannabidiol at the cellular level in stressed models. The SKN-1 gene, the Nrf homolog in C. elegans, encodes for three different isoforms, skn-1a, skn-1b, and c. Skn-1b/c, which plays a role in oxidative stress, is negatively regulated by the repressor WDR-23. In C. elegans, skn-1a plays a role in proteotoxic stress through upregulation proteosome subunits and is negatively regulated by the abundance of proteosome complex protein. Results show that 10μM of CBD was able to activate isoforms of skn-1, skn-1a and skn-1b/c. The ROS scavenging activity of CBD was dependent on the presence of skn-1b/c. Furthermore, CBD's protective effects under proteotoxic stress were diminished in the absence of skn-1a. Further investigation will be conducted to identify the role of skn-1 in CBD's reduction of Aβ plaques. | |
| dc.format.medium | born digital | |
| dc.format.medium | masters theses | |
| dc.identifier | Alsulami_colostate_0053N_17672.pdf | |
| dc.identifier.uri | https://hdl.handle.net/10217/236590 | |
| dc.language | English | |
| dc.language.iso | eng | |
| dc.publisher | Colorado State University. Libraries | |
| dc.relation.ispartof | 2020- | |
| dc.rights | Copyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright. | |
| dc.title | Skn-1, Nrf homolog, mediates cannabidiol cellular stress responsive effects in Caenorhabditis elegans | |
| dc.type | Text | |
| dcterms.rights.dpla | This Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). | |
| thesis.degree.discipline | Environmental and Radiological Health Sciences | |
| thesis.degree.grantor | Colorado State University | |
| thesis.degree.level | Masters | |
| thesis.degree.name | Master of Science (M.S.) |
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