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The DXO decapping exonuclease is a restriction factor for RNA viruses

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dc.contributor.authorLynch, Erin R., author
dc.contributor.authorGeiss, Brian, advisor
dc.contributor.authorWilusz, Jeffrey, committee member
dc.contributor.authorPerera, Rushika, committee member
dc.contributor.authorStasevich, Tim, committee member
dc.date.accessioned2020-01-13T16:41:41Z
dc.date.available2020-01-13T16:41:41Z
dc.date.issued2019
dc.description.abstractCellular RNA exonucleases, such as XRN1 and DXO, aid in the destruction of defective cellular mRNAs and help maintain overall cellular health. The RNA decay system, however, also serves another purpose – degrading viral RNAs. The XRN1 exonuclease is known to be a major antagonist of RNA virus genomes, but the role of other cellular RNA decay enzymes in controlling viral infection is less clear. The cellular 5' decapping exonuclease DXO is able to recognize, de-cap, and degrade RNAs lacking 2'-O-methylation on the first nucleotide after the 5' cap, helping the cell to discriminate self from non-self RNAs. Preliminary data we have developed indicate that flaviviruses and alphaviruses replicate to much higher levels in DXO deficient cells than in cells containing DXO, indicating that DXO may also act as a cellular viral restriction factor. Interestingly, flavivirus genomes contain a 5' cap that is generally 2'-O-methylated at the first base of the transcript, providing a potential mechanism to evade DXO degradation. Overall, our results indicate that the DXO decapping exonuclease helps control the replication of positive strand RNA viruses in cells and represents a new viral restriction factor.
dc.format.mediumborn digital
dc.format.mediummasters theses
dc.identifierLynch_colostate_0053N_15745.pdf
dc.identifier.urihttps://hdl.handle.net/10217/199776
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado State University. Libraries
dc.relation.ispartof2000-2019
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.
dc.subjectantiviral
dc.subjectflavivirus
dc.subjectalphavirus
dc.subjectRNA decay
dc.subjectDXO
dc.titleThe DXO decapping exonuclease is a restriction factor for RNA viruses
dc.typeText
dcterms.rights.dplaThis Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
thesis.degree.disciplineCell and Molecular Biology
thesis.degree.grantorColorado State University
thesis.degree.levelMasters
thesis.degree.nameMaster of Science (M.S.)

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