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Phylogenetic analysis of Xanthomonas translucens & genome-enabled diagnostic tools for pathogens of cereals and non-cereals

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dc.contributor.authorLanglois, Paul, author
dc.contributor.authorLeach, Jan, advisor
dc.contributor.authorJahn, Courtney, committee member
dc.contributor.authorSteingraeber, David, committee member
dc.date.accessioned2016-07-13T14:50:22Z
dc.date.available2017-07-12T06:30:24Z
dc.date.issued2016
dc.description.abstractPrevalence of Xanthomonas translucens, which causes bacterial leaf streak (BLS) in cereal crops and bacterial wilt in forage and turfgrass species, has increased in many regions in recent years. Because the pathogen is seedborne in economically important cereals, it is a concern for international and interstate germplasm exchange, and thus, reliable and robust protocols for its detection in seed are needed. However, historical confusion in the taxonomy within the species has complicated the development of accurate and reliable diagnostic tools for X. translucens. The goal of this study was to clarify the genetic relationships of X. translucens pathovars, and to use that information to develop useful and robust diagnostic tools. We sequenced genomes of 15 X. translucens isolates representing six different pathovars. Based on Multilocus Sequence Typing (MLST), wheat isolates designated as X. translucens pv. undulosa are in the same phylogenetic clade as barley isolates identified as X. translucens pv. translucens. The wheat and barley pathovars, undulosa and translucens, are genetically distinct from the cerealis pathovar isolated from either cereals or non-cereals, as well as pathovars isolated from other non-cereals, including arrhenatheri, graminis, and poae. Using unique genomic regions, Loop Mediated Isothermal Amplification (LAMP) primer sets were designed that selectively amplified X. translucens (species-specific), or that selectively amplified strains belonging to cerealis and poae pathovars. In addition, LAMP PCR assays were developed that distinguished X. translucens strains associated with cereal leaf streak (CLS), such as undulosa, translucens, hordei, and secalis, from the other cereal or non-cereal pathovars.
dc.format.mediumborn digital
dc.format.mediummasters theses
dc.identifier.urihttp://hdl.handle.net/10217/173511
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado State University. Libraries
dc.relation.ispartof2000-2019
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.
dc.subjectbarley
dc.subjectLAMP
dc.subjectwheat
dc.subjectdiagnostics
dc.subjectbacteria
dc.subjectphylogenetics
dc.titlePhylogenetic analysis of Xanthomonas translucens & genome-enabled diagnostic tools for pathogens of cereals and non-cereals
dc.typeText
dcterms.embargo.expires7/12/2017
dcterms.embargo.terms7/12/2017
dcterms.rights.dplaThis Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
thesis.degree.disciplineBioagricultural Sciences and Pest Management
thesis.degree.grantorColorado State University
thesis.degree.levelMasters
thesis.degree.nameMaster of Science (M.S.)

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