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Serial protein misfolding cyclic amplification (sPMCA) to detect surrogate markers for chronic wasting disease in surface water, municipal water and soil

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dc.contributor.authorNichols, Tracy A., author
dc.contributor.authorZabel, Mark, advisor
dc.date.accessioned2024-03-13T20:12:28Z
dc.date.available2024-03-13T20:12:28Z
dc.date.issued2008
dc.description.abstractChronic wasting disease (CWD) is a transmissible spongiform encephalopathy of deer and elk. Research has indicated that CWD is transmitted horizontally, and that both blood and saliva can transmit disease. Environmental exposure to pens where infected animals have been kept has resulted in disease transmission to deer. However, examination of environmental components such as soil and water for prions has been hampered by sensitivity limitations of conventional western blotting and inoculation limitations of bioassays. In this study we evaluated the ability of protein misfolding cyclic amplification (PMCA) to detect protease-resistant prion protein (PrPres) in environmental samples such as water and soil. Serial protein misfolding cyclic amplification (sPMCA) of PrPres, the misfolded proteinase-resistant protein associated with prion disease, was used to detect prion-infected brain homogenate spiked into soil and water to determine detection limits of this assay in environmental samples. The PrPres detection limit for water after 6 rounds of PMCA was 1:26 x 106. Detection of a CWD spike in soil with our current methodology was not possible. We next evaluated surface and drinking water from a CWD endemic region of Colorado for PrPres by sPMCA. PrPres was detected in Cache la Poudre River and flocculant samples at a time of high snowmelt runoff, suggesting that sPMCA can be a useful tool in evaluating water for PrP res in CWD-endemic areas.
dc.format.mediumborn digital
dc.format.mediumdoctoral dissertations
dc.identifierETDF_Nichols_2008_3346467.pdf
dc.identifier.urihttps://hdl.handle.net/10217/237890
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado State University. Libraries
dc.relation.ispartof2000-2019
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.
dc.rights.licensePer the terms of a contractual agreement, all use of this item is limited to the non-commercial use of Colorado State University and its authorized users.
dc.subjectchronic wasting disease
dc.subjectenvironmental contamination
dc.subjectprions
dc.subjectserial protein misfolding cyclic amplification
dc.subjectsoil
dc.subjectsurrogate markers
dc.subjecttransmission
dc.subjectwater
dc.subjectmolecular biology
dc.subjectmicrobiology
dc.subjectanimal sciences
dc.subjectanimal diseases
dc.titleSerial protein misfolding cyclic amplification (sPMCA) to detect surrogate markers for chronic wasting disease in surface water, municipal water and soil
dc.typeText
dcterms.rights.dplaThis Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
thesis.degree.disciplineCell and Molecular Biology
thesis.degree.grantorColorado State University
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Philosophy (Ph.D.)

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