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Laboratory evaluation of a microfluidic electrochemical sensor for aerosol oxidative load

dc.contributor.authorShapiro, Jeffrey, author
dc.contributor.authorVolckens, John, advisor
dc.contributor.authorHenry, Charles, committee member
dc.contributor.authorPeel, Jennifer, committee member
dc.description.abstractHuman exposure to particulate matter (PM) air pollution is associated with both human morbidity and mortality. The mechanisms by which PM impacts human health are yet unresolved, but evidence suggests that PM intake leads to cellular oxidative stress through the generation of reactive oxygen species (ROS). Therefore, reliable tools are needed for estimating the oxidant generating capacity, or oxidative load, of PM. The most widely reported method for assessing PM oxidative load is the dithiothreitol (DTT) assay. The traditional DTT assay utilizes filter-based PM collection in conjunction with laboratory analysis. However, the traditional DTT assay suffers from poor time resolution, loss of reactive species during sampling, and high limit of detection. Recently, a new DTT assay was developed by coupling a Particle Into Liquid Sampler with microfluidic-electrochemical detection. This 'on-line' system allows continuous monitoring of PM reactivity (~three minute measurement resolution) from substantially reduced sample masses (nanograms). This study reports on a laboratory evaluation of the on-line DTT approach. A standard urban dust sample was aerosolized in a laboratory test chamber at three atmospherically-relevant concentrations allowing comparison of the on-line and traditional DTT methods. The on-line system gave a stronger correlation between DTT consumption rate and PM mass (R2 = 0.93) than the traditional method (R2 = 0.29). The on-line system also reported ~1.4 times greater relative reactivity for a given PM sample compared to the traditional method (p = 0.022) indicating improved efficiency for the capture and detection of redox-active species. These results suggest that on-line methods for PM sampling and reactivity analysis may improve our ability to study impacts of PM exposure on human health.
dc.format.mediumborn digital
dc.format.mediummasters theses
dc.publisherColorado State University. Libraries
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see
dc.subjectoxidative stress
dc.subjectparticulate matter
dc.subjectreactive oxygen species
dc.titleLaboratory evaluation of a microfluidic electrochemical sensor for aerosol oxidative load
dcterms.rights.dplaThis Item is protected by copyright and/or related rights ( You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). and Radiological Health Sciences State University of Science (M.S.)


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