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Influence of polymeric nanowire topography on the differentiation of adipose-derived stem cells

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dc.contributor.authorTrujillo, Nathan Anthony, author
dc.contributor.authorPopat, Ketul, advisor
dc.contributor.authorWilliams, John, committee member
dc.contributor.authorDasi, Lakshmi Prasad, committee member
dc.contributor.authorKipper, Matt, committee member
dc.date.accessioned2007-01-03T06:35:06Z
dc.date.available2007-01-03T06:35:06Z
dc.date.issued2014
dc.description.abstractConsidering the many advances in tissue engineering, there are still significant challenges associated with restructuring, repairing, or replacing damaged tissue in the body. Recently biodegradable synthetic scaffolds have shown to be a promising alternative. However, based on the application, it is essential that the scaffold possess specific surface properties that promote cell-scaffold interactions and aid in extracellular matrix deposition. In this work, we present a novel solvent-free, template-synthesis technique for creating substrate-bound nanowire scaffolds from polycaprolactone, a biocompatible and biodegradable polymer. Nanowire surfaces were also fabricated from polycaprolactone that included 1 wt% hydroxyapatite nanoparticles for osteogenic studies. The fundamental concept behind successful synthetic tissue-engineered scaffolds is to promote progenitor cell migration, adhesion, proliferation, and induce differentiation, extracellular matrix synthesis, and finally integration with host tissue. Recently, lipoaspirate tissue has been identified as a viable alternative source for mesenchymal stem cells because it contains a supportive stroma that can easily be isolated. Adipose derived stem cells can differentiate into a variety of mesodermal lineages including the osteogenic, chondrogenic, and adipogenic phenotypes. The results indicated that during the growth period i.e., initial 7 days of culture, the nanowire surfaces supported adhesion, proliferation, and viability of the cells in addition to morphological changes. Osteogenic, chondrogenic, and adipogenic differentiation potential of adipose derive stem cells was evaluated with and without differentiation supplements to determine the influence of nanowire architecture on mechanotrasnduction. It was determined that nanowire topography stimulated the expression of osteogenic marker proteins, osteocalcin and osteopontin, as well as mineralization and alkaline phosphatase activity.
dc.format.mediumborn digital
dc.format.mediumdoctoral dissertations
dc.identifierTrujillo_colostate_0053A_12631.pdf
dc.identifier.urihttp://hdl.handle.net/10217/83824
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado State University. Libraries
dc.relation.ispartof2000-2019
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.
dc.subjectadipogenic
dc.subjectchondrogenic
dc.subjectnanowires
dc.subjectosteogenic
dc.subjectpolycaprolactone
dc.subjectstem cells
dc.titleInfluence of polymeric nanowire topography on the differentiation of adipose-derived stem cells
dc.typeText
dcterms.rights.dplaThis Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
thesis.degree.disciplineMechanical Engineering
thesis.degree.grantorColorado State University
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Philosophy (Ph.D.)

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