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Quantitative analysis of the mechanical environment in the embryonic heart with respect to its relationship in cardiac development

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dc.contributor.authorBulk, Alexander T., author
dc.contributor.authorDasi, Lakshmi Prasad, advisor
dc.contributor.authorGarrity, Deborah, advisor
dc.contributor.authorPopat, Ketul, committee member
dc.contributor.authorOrton, Christopher, committee member
dc.date.accessioned2016-01-11T15:14:08Z
dc.date.available2016-01-11T15:14:08Z
dc.date.issued2015
dc.description.abstractIn order to understand the causes of congenital heart defects, which afflict at least 4 infants per 1,000 live births, research has implemented the use of animal models to study embryonic heart development. Zebrafish (Danio rerio) have become one of the more prominent of these animal models due to the fact that their heart morphology at the earliest stages of development is remarkably similar to humans, and because embryos lack pigmentation, rendering them transparent. This transparency allows for high-speed images of blood flow to be acquired in the developing heart so that the mechanotransductive relationship between the intracardiac flow environment and myocardial progenitor cell differentiation can be understood. One particular aspect of the flow environment, a cyclic retrograde flow at the junction of the forming atrium and ventricle, has been shown to be necessary for valve formation, though the mechanisms causing it to occur had previously been unknown. By comparing the results of two-dimensional spatiotemporal analysis applied to embryos both with normal retrograde flow and inhibited retrograde flow, this study shows that a particular range of pressures associated with the pumping mechanics of the heart as well as resistance due to systolic contractile closure must exist in order to maintain adequate retrograde flow to induce valve formation. The use of two-dimensional spatiotemporal analysis was sufficient to acquire these results, however when applied to analysis of other aspects of the intracardiac flow environment, this computational method is subject to critical limitations. Therefore, this study includes the development of methodology to integrate the results of spatiotemporal analysis on multiple focal planes bisecting the heart into a more accurate, three-dimensional result. The results of this study not only increase our understanding of the mechanics behind an important factor in embryonic development, but also enable future experiments pertaining to the measurement of embryonic intracardiac blood flow to be performed with increased certainty.
dc.format.mediumborn digital
dc.format.mediummasters theses
dc.identifierBulk_colostate_0053N_13415.pdf
dc.identifier.urihttp://hdl.handle.net/10217/170434
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado State University. Libraries
dc.relation.ispartof2000-2019
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.
dc.subjectembryonic
dc.subjectfocal plane
dc.subjectheart development
dc.subjectmechanotransduction
dc.subjecttranscription
dc.subjectzebrafish
dc.titleQuantitative analysis of the mechanical environment in the embryonic heart with respect to its relationship in cardiac development
dc.typeText
dcterms.rights.dplaThis Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
thesis.degree.disciplineMechanical Engineering
thesis.degree.grantorColorado State University
thesis.degree.levelMasters
thesis.degree.nameMaster of Science (M.S.)

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