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T cell mediated satellite cell function: implications for age-associated changes in skeletal muscle regeneration

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dc.contributor.authorDumke, Breanna R., author
dc.contributor.authorLees, Simon J., advisor
dc.contributor.authorGotshall, Robert W., advisor
dc.contributor.authorFrye, Melinda A., committee member
dc.date.accessioned2007-01-03T04:51:31Z
dc.date.available2007-01-03T04:51:31Z
dc.date.issued2010
dc.description.abstractSarcopenia is an age-associated loss of skeletal muscle mass and strength. Recent evidence suggests that an age-associated loss of muscle precursor cell (MPC) functionality contributes to sarcopenia. Current research also suggests that T cells of the immune system may influence skeletal muscle repair via signaling with MPCs. The objective of the present study was to examine the influence of activated T cells on MPCs. MPCs were collected from the gastrocnemius and plantaris from 3-mo-old (young) and 32-mo-old (old) animals. Splenic T cells were also harvested using anti-CD3 Dynabead isolation. T cells were activated for 48 hours with co-stimulation of 100 IU/ml Interleukin-2 (IL-2) and 5 ug/ml of anti-CD28. Co-stimulation increased 5-bromo-2'-deoxyuridine (BrdU) incorporation (proliferation) of T cells from 13.382% (SEM=4.55, n=5) in control to 64.77% (SEM= 6.02, n=5). Additionally, T cell cytokines increased MPC proliferation by 23.98% (SEM=5.69, n=4) in young MPCs but decreased by 1.58% (SEM=4.09, n=4) in old MPCs. T cell cytokines were also found to be chemoattractant. Young MPCs migrated at a rate of 1.36 (SEM=0.56, n=4) with T cell cytokines. Old MPCs, however, did not migrate with T cell cytokines -0.05 (SEM= 0.214, n=4). These data suggest that T cells may play a critical role in mediating MPC function. Furthermore, aging may alter T cell-induced MPC function. These findings have implications for developing strategies aimed at increasing MPC proliferation and the regenerative capacity of aged skeletal muscle.
dc.format.mediummasters theses
dc.identifier2010_Fall_Dumke_Brianna.pdf
dc.identifierETDF2010300010HAES
dc.identifier.urihttp://hdl.handle.net/10217/44849
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado State University. Libraries
dc.relation.ispartof2000-2019
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.
dc.subjectT cell
dc.subjectskeletal muscle
dc.subjectsatellite cell
dc.subjectproliferation
dc.subjectmigration
dc.subjectconditioned media
dc.subject.lcshMuscles -- Diseases
dc.subject.lcshMuscles -- Regeneration
dc.subject.lcshT cells -- Therapeutic use
dc.subject.lcshMuscles -- Diseases
dc.titleT cell mediated satellite cell function: implications for age-associated changes in skeletal muscle regeneration
dc.typeText
dcterms.rights.dplaThis Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
thesis.degree.disciplineHealth and Exercise Science
thesis.degree.grantorColorado State University
thesis.degree.levelMasters
thesis.degree.nameMaster of Science (M.S.)

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