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The extraction and measurement of adenosine triphosphate from marine sediment

Date

1976-05

Authors

Paul, E. A., author
Bancroft, K., author
Wiebe, W. J., author
American Society of Limnology and Oceanography, publisher

Journal Title

Journal ISSN

Volume Title

Abstract

A technique has been developed, using boiling sodium bicarbonate buffer, to extract adenosine triphosphate (ATP) from marine sediments and has been tested on a variety of sediments, including those with high organic content, clay, and carbonate. Recovery of ATP, as measured by the addition of bacteria of known ATP content to sediment, varied from 64-100%. The technique also was as effective as the conventional Tris buffer for extraction of ATP from both pure cultures of bacteria grown in broth and natural seawater samples.

Description

Publisher version: http://www.jstor.org/stable/2835285.

Rights Access

Subject

extraction
ATP standards
sediment
ATP technique
adsorptive characteristics
biomass

Citation

Associated Publications