Extraction of free amino acids from soil
Alternatives to extraction with ethanol were examined in order to obtain more effective removal of free amino acids from soil. Ba(OH)2 was a promising extractant and proved to be very effective in recovering a mixture of 17 amino acids that had been added to soil. Resolution of the extract by elution chromatography was followed by colorimetric analysis; 73 to 121% of the acidic and neutral components of the mixture and 36 to 41% of the basic amino acids were recovered. A second extraction procedure, based on the use of NH4OAc, was developed to avoid any slight hydrolysis of amino acid polymers ...
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