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dc.contributor.authorHarris, D.
dc.contributor.authorVoroney, R. P.
dc.contributor.authorPaul, Eldor A.
dc.date.accessioned2007-01-03T06:07:11Z
dc.date.available2007-01-03T06:07:11Z
dc.date.issued1997-11
dc.descriptionIncludes bibliographical references (page 514).
dc.description.abstractWe present a calculation for soil microbial biomass N:C ratio determined from a 10-d incubation following chloroform fumigation. The calculation is based on a mathematical model of the N content of the pre- and post-fumigation soil microbial biomass and the growth yield of the biomass that develops after fumigation. Biomass N is calculated from the N:C ratio and biomass C. The mineralization of bacteria and fungi, with different N contents, added to fumigated soils was used to establish the model parameters. The model was tested against an independent set of measurements and considers two assumptions: 1) The ratio of N:C mineralized from killed biomass is equal to the ratio of N:C mineralized from soil non-biomass constituents. 2) More realistically, the N and C mineralization in the fumigated soil, from sources other than killed biomass, is a residual fraction of the N and C mineralization in the unfumigated soil. Biomass C:N ratios calculated without a control correction (assumption 1) were, on average, 20% wider than corrected values (assumption 2). Biomass N calculated as the product of N:C and biomass C was compared with published values for several data sets. The new calculation method was robust even when net immobilization of N followed fumigation.
dc.format.mediumborn digital
dc.format.mediumarticles
dc.identifier.bibliographicCitationHarris, D., R. P. Voroney and E. A. Paul, Measurement of Microbial Biomass N:C by Chloroform Fumigation-Incubation. Canadian Journal of Soil Science 77, no. 4 (November 1997): 507-514. https://dx.doi.org/10.4141/S96-064.
dc.identifier.doihttps://dx.doi.org/10.4141/S96-064
dc.identifier.urihttp://hdl.handle.net/10217/80890
dc.languageEnglish
dc.publisherColorado State University. Libraries
dc.publisher.originalAgricultural Institute of Canada
dc.relation.ispartofFaculty Publications - Natural Resource Ecology Laboratory (NREL)
dc.rights©1997 Agricultural Institute of Canada
dc.subjectsoil microbial biomass
dc.subjectC:N ratio
dc.subjectchloroform fumigation
dc.subjectnitrogen
dc.titleMeasurement of microbial biomass N:C by chloroform fumigation-incubation
dc.typeText


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