Variation in chromosomal aberration induction in differing ATM and ASPM genotypic backgrounds detected via a modified PNA FISH technique
A modified PNA (peptide nucleic acid) FISH (fluorescence in situ hybridization) method has been developed for efficient quantitative and qualitative analysis of chromosomal aberration formation. PNA FISH was used to detect a heightened sensitivity in mouse fibroblasts in response to ionizing radiation with Atm-/- and in mouse embryonic fibroblasts (MEFs) in response to loss of Aspm gene respectively. For Atm analysis, four commonly used inbred mouse strains were used, C57BL/6J, A/J, 129S6, and BALB/cByJ, and exposed to cesium-137 at a dose rate of approximately 2.5 Gy/min. For Aspm analysis, ...
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