New methods for cryopreserving rooster spermatozoa
Rooster spermatozoa sustain permanent membrane damage during cooling, thawing and dilution due to inappropriate changes during the phase transition and osmotic intolerance. Glycerol is a common cryoprotectant used to protect cells during freezing, but its relatively large molecular weight and relatively low membrane permeability (compared to water) creates relatively long aniosmotic conditions when glycerol is added or removed. Glycerol-exposed rooster spermatozoa exhibit poor fertility, therefore, sperm must undergo an extensive dilution and sperm reconcentration process to remove glycerol and ...
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