DNA strand break associated bystander effect (DSB-ABE) is linked to gene mutations in naïve cells and indicates the involvement of a MAPK pathway
The goal of this project was to investigate whether radiation independent DNA damage, specifically a single induced DNA double strand break (DSB) can produce bystander signal, which is capable of inducing genomic instability in non-targeted cells. Previously uncharacterized E18 (modified TK6 cells) with a unique I-Sce1 insert in intron 2 of the thymidine kinase (TK1) gene were allowed to generate a bystander signal following electroporation of the rare cutting restriction enzyme I-Sce1 carried by a plasmid to induce DNA damage at the I-Sce1 site. Mutation assays were carried out to measure ...
(For more, see "View full record.")