Comparison of the limulus amebocyte lysate (LAL) and recombinant factor C (rFC) endotoxin bioassays: characterization of aerosols and settled dust on a sheep farm
The goals of this study are: 1) To optimize an extraction protocol for bulk sheep dust analysis by rFC; 2) To compare the traditional chromogenic LAL to the newer fluorometric rFC assay; 3) To compare the effects of the two most widely used extraction media, pyrogen-free water (PFW) and pyrogen-free water with 0.05% Tween 20 (PFW-Tween 20), on the assay outcomes; and 4) To characterize endotoxin exposure in a sheep farm environment. Settled and airborne dust samples were collected from a sheep farm for analysis. Settled dust was analyzed with endpoint fluorogenic rFC and kinetic chromogenic ...
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