Dataset associated with “Using isotope pool dilution to understand how organic carbon additions affect N2O consumption in diverse soils”
Date
2022
Authors
Stuchiner, Emily R.
von Fischer, J. C.
Journal Title
Journal ISSN
Volume Title
Abstract
Nitrous oxide (N2O) is a formidable greenhouse gas with warming potential ~300x
greater than CO2. However, its emissions to the atmosphere have gone largely unchecked
because the microbial and environmental controls governing N2O emissions have proven
difficult to manage. The microbial process N2O consumption is the only know biotic pathway to
remove N2O from soil pores and therefore reduce N2O emissions. Consequently, manipulating
soils to increase N2O consumption by organic carbon (OC) additions has steadily gained interest.
However, the response of N2O emissions to different OC additions are inconsistent, and it is
unclear if lower N2O emissions are due to increased consumption, decreased production, or both.
Simplified and systematic studies are needed to evaluate the efficacy of different OC additions
on N2O consumption. We aimed to manipulate N2O consumption by amending soils with OC compounds
(succinate, acetate, propionate) more directly available to denitrifiers. We hypothesized that N2O
consumption is OC-limited and predicted these denitrifier-targeted additions would lead to
enhanced N2O consumption and increased nosZ gene abundance. We incubated diverse soils in
the laboratory and performed a 15N2O isotope pool dilution assay to disentangle microbial N2O
emissions from consumption using laser-based spectroscopy. We found that amending soils with
OC increased gross N2O consumption in six of eight soils tested. Furthermore, three of eight
soils showed Increased N2O Consumption and Decreased N2O Emissions (ICDE), a
phenomenon we introduce in this study as an N2O management ideal. All three ICDE soils had
low soil OC content, suggesting ICDE is a response to relaxed C-limitation wherein C additions
promote soil anoxia, consequently stimulating the reduction of N2O via denitrification. We
suggest, generally, OC additions to low OC soils will reduce N2O emissions via ICDE. Future
studies should prioritize methodical assessment of different, specific, OC-additions to determine
which additions show ICDE in different soils.
Description
This dataset includes information about eight soils collected from Colorado, New Mexico, and Minnesota. All soils were collected from June – July 2019. Five soils were collected in Colorado (two in Rocky Mountain National Park, one from a cornfield near Greeley, CO, one from a grassland near Nunn CO, and one from a lawn at Colorado State University). One soil was collected in New Mexico, from the Sevilleta National Grassland. Two soils were collected from Minnesota (one from a coniferous forest near Ely, MN, and one from a cornfield near Elrosa, MN). We subsampled these soils to analyze their soil chemical and microbial genetic properties, and we also subsampled these soils to analyze their nitrous oxide (N2O) emission and isotopic composition. The purpose of these analyses was to determine how amending soils with organic carbon influenced their capacity to produce and consume N2O. All of these analyses occurred at Colorado State University, from July – September 2019.
Department of Biology
Graduate Degree Program in Ecology
Department of Biology
Graduate Degree Program in Ecology
Rights Access
Subject
Soil N2O emission
Stimulate N2O consumption
Organic carbon amendments
Soil properties
nosZ gene abundance
15N isotope pool dilution
Microbial N-cycling genes
Citation
Associated Publications
Stuchiner, E. R., & von Fischer, J. C. (2022). Using isotope pool dilution to understand how organic carbon additions affect N2O consumption in diverse soils. Global Change Biology, 00, 1– 17. https://doi.org/10.1111/gcb.16190