Estimating potential biosafety risk of a CRISPR-Cas9 system for targeted killing of certain pathogens in beef cattle production using omic-based analysis methodologies
The CRISPR-Cas9 system has emerged as a programmable and versatile tool for precise gene editing purposes. In addition to gene editing, the CRISPR-Cas9 system can be developed to kill targeted bacteria. In our previous studies, we developed a CRISPR-Cas9 targeted killing system with a guide RNA designed to specifically recognize the Shiga-toxic genes (stx1 and stx2). Delivery of this system into E. coli cells could effectively kill Shiga-toxin producing E. coli (STEC) cells. This current study was conducted to estimate potential biosafety risk associated with our CRISPR-Cas9-based targeted killing ...
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