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dc.contributor.advisorJackson, Mary
dc.contributor.authorWiersma, Crystal J.
dc.contributor.committeememberBorlee, Bradley
dc.contributor.committeememberJahn, Courtney
dc.date.accessioned2020-01-13T16:42:02Z
dc.date.available2020-01-13T16:42:02Z
dc.date.issued2019
dc.description2019 Fall.
dc.descriptionIncludes bibliographical references.
dc.description.abstractMycobacterium abscessus complex is a group of rapid-growing nontuberculous mycobacteria that are multidrug resistant and that cause chronic pulmonary infections in individuals with cystic fibrosis (CF) and other pre-disposing conditions. Research progress is challenged by the lack of laboratory models that mimic the lung environment, a nutritionally complex environment not well represented by current laboratory medium. In this study, the growth characteristics and gene expression profile of a diverse panel of M. abscessus isolates were characterized and compared when grown in 7H9 Middlebrook medium (a synthetic Mycobacterium laboratory culture medium); Synthetic Cystic Fibrosis Medium 2 (SCFM2), a medium which mimics the composition of CF sputum; and actual patient CF sputum. Tests were also performed measuring the antibiotic susceptibility and characterizing the cell envelope composition of the M. abscessus isolates in these media. Although the medium composition did not affect the antibiotic susceptibility or growth of the isolates, it caused changes in fatty acid and outer membrane lipid compositions which may account, at least in part, for observed differences in the subsequent infectivity of the isolates inside macrophages and epithelial cells. The gene expression profiles showed similar upregulation of pathways related to carbon metabolism for Mycobacterium abscessus grown in SCFM2 and grown in CF sputum while reaffirming the complexity of CF sputum and the many metabolic and structural adaptations that M. abscessus undergoes during growth in varied environments.
dc.format.mediumborn digital
dc.format.mediummasters theses
dc.identifierWiersma_colostate_0053N_15809.pdf
dc.identifier.urihttps://hdl.handle.net/10217/199831
dc.languageEnglish
dc.publisherColorado State University. Libraries
dc.relation.ispartof2000-2019 - CSU Theses and Dissertations
dc.rightsCopyright of the original work is retained by the author.
dc.subjectcystic fibrosis
dc.subjectmycobacteria
dc.subjectphysiology
dc.subjectmodel
dc.subjectabscessus
dc.subjectnontuberculous
dc.titleComparative physiology of Mycobacterium abscessus in synthetic laboratory medium and cystic fibrosis sputum
dc.typeText
dcterms.rights.dplaThe copyright and related rights status of this Item has not been evaluated (https://rightsstatements.org/vocab/CNE/1.0/). Please refer to the organization that has made the Item available for more information.
thesis.degree.disciplineMicrobiology, Immunology, and Pathology
thesis.degree.grantorColorado State University
thesis.degree.levelMasters
thesis.degree.nameMaster of Science (M.S.)


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