Towards macromolecular scaffold assisted crystallography
The current, dominant method for structure determination in atomic detail is X-ray crystallography; but, this method requires a brute force search through non-physiological solution conditions looking for the "needle-in-a-haystack" condition in which the target protein crystallizes. Unfortunately, despite exhaustive screening, most proteins of interest do not form crystals. Other proteins are difficult to obtain in sufficient quantities to make the attempt. Finally, even successful crystals reveal a structure adopted under artificial conditions-a single snapshot that dramatically underrepresents ...
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