2005 Projects

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Open Access
Evaluation of Aedes aegypti densonucleosis (AeDNV) infection in adult mosquito mortality
(Colorado State University. Libraries, 2005) Plake, Emily M., author; Doehling, Malena, author; Bray, Stacey, author; Carlson, Jon, author; Suchman, Erica, author
Aedes aegypti densonucleosis virus (AeDNV) has long been considered a likely agent in biologic control against arthropod-borne diseases. Extensive studies have been done to evaluate infection and pathogeneses in Aedes aegypti larvae and pupae, but only primary studies have been preformed in adults. The primary studies on adult mortality produced a two part mortality curve that showed high levels of early mortality, low levels of intermediate mortality and high levels of late mortality. It was hypothesized that the high levels of early mortality were due to high levels of virus infection, whereas the high levels late mortality were due to old age. In this study we analyzed the mosquitoes that died early and late in the mortality assay with quantitative PCR to determine virus titers in individual mosquitoes. We found that there is no significant difference between titer level of mosquitoes that died in the early part of the curve (Day 17-29), which had an average of 1.264 x 1011 genome equivalents per ml and those that died in the late part of the curve (Day 45-54), which had an average of 2.255 x 1011 genome equivalents per ml.