Browsing by Author "Angeloni, Lisa, advisor"

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Open Access
Enrichment as a conservation tool to enhance behavior, morphology, gene expression, and survival in Arkansas darters
(Colorado State University. Libraries, 2023) Kopack, Christopher J., author; Angeloni, Lisa, advisor; Fetherman, Eric, advisor; Ghalambor, Cameron, committee member; Kanno, Yoichiro, committee member
Conservation practitioners often rely on captive breeding programs to supplement wild populations at risk of extinction. While population augmentation has been successful for some taxa, the use of hatchery fish to supplement wild populations can be severely impacted by predation. Elevated predation on hatchery fish may arise because hatchery environments often differ starkly from wild environments, constraining the ability of hatchery fish to phenotypically match the environments in which they are targeted for release. Phenotypic mismatch caused by differences between hatchery and wild environments can limit efforts to conserve fish species at risk of extinction when hatchery-reared fish are used to augment wild populations. Phenotypes adapted to or induced by hatchery environments are thought to be maladapted for life in the wild. Thus, enriching the hatchery environment (abiotically and biotically) to make it more similar to the wild may induce phenotypes, including behavior, morphology, and gene expression profiles, that are better suited to the environments fish will experience after release. Chapter One explores how hatchery-reared fish respond to novel predators and whether those responses can be enhanced to improve survival. Identifying the presence of innate predator recognition and the capacity for learning to recognize predators can inform conservation management practices. We assessed antipredator behavior (time spent moving and distance from a predator) and the efficacy of predator training for three populations of a species of conservation concern, the Arkansas darter (Etheostoma cragini), which is vulnerable to predation by esocid predators like the introduced northern pike (Esox lucius). Arkansas darters demonstrated an innate ability to recognize and respond to a novel esocid predator. Their behavior also changed in response to predator cues (training), though the direction of response to cues was opposite our prediction. Populations differed in their response to the predator treatment, highlighting the potential value of managing populations separately. Our results suggest that antipredator behavior is innate and that exposure to predator cues does affect behavior. This study demonstrates the importance of evaluating enrichment practices and incorporating behavioral observations into conservation programs to guide population-specific management decisions. In Chapter Two, we used a factorial approach to assess whether abiotic enrichment and biotic enrichment (predator recognition training) increase survival of Arkansas darters during encounters with a novel predator. We also assessed the effects of abiotic enrichment on the expression of behavioral and morphological phenotypes across three populations. Morphology and behavior differed among populations and between abiotic treatments, and populations responded differently to the abiotic treatments. Furthermore, we found that in combination with predator training, abiotic enrichment increased the probability of surviving a first encounter with a predator. We therefore recommend conservation practitioners incorporate abiotic enrichment and predator recognition training in the hatchery, as any increase in survival is expected to benefit efforts to conserve this species. In Chapter Three, we took a molecular approach (TagSeq) to elucidate how abiotic enrichment and biotic enrichment impacts the whole-brain gene expression of Arkansas darters, comparing the effects in two hatchery populations to a wild reference population. Although, we found no effect of biotic enrichment on gene expression, we did find that abiotic enrichment has the potential to reduce phenotypic mismatch between hatchery and wild fish, indicating that enrichment may aid current conservation efforts. Overall, these studies suggest a potential role for enrichment in the conservation of imperiled fish, and they highlight the value of a phenotypic approach to managing populations.
Open Access
Experimental tests of risky augmentation scenarios using Trinidadian guppies
(Colorado State University. Libraries, 2017) Kronenberger, John Andrew, author; Angeloni, Lisa, advisor; Funk, Chris, advisor; Ghalambor, Cameron, committee member; Hufbauer, Ruth, committee member
Increased isolation of populations, and the subsequent reduction in genetic diversity, can exacerbate global biodiversity loss by contributing to inbreeding depression and reducing the ability of organisms to adapt to rapid environmental change. This has prompted some conservation biologists to consider augmenting isolated populations with immigrants as a means of demographic and genetic rescue. Augmentations are typically highly successful, but they are also controversial due to the risk of outbreeding depression or the introduction of maladapted alleles when immigrants are genetically or adaptively divergent. For my Master's thesis, I tested risky augmentation scenarios using mesocosm populations of Trinidadian guppies (Poecilia reticulata) in two separate controlled experiments. In my first experiment (Chapter 1), I augmented mesocosm populations derived from a single recipient source with genetically or adaptively divergent immigrants to assess their short-term demographic effects. Mesocosms that were augmented maintained greater abundance and recruitment than controls that were not. There was also a trend for populations to receive a greater benefit from immigrants that were genetically divergent than those that were adaptively divergent. I expanded upon these results in my second experiment (Chapter 2), in which I augmented mesocosm populations from two different recipient sources with immigrants spanning a greater range of divergence and monitored them over a longer time frame, including an additional control and genetic monitoring to determine the relative impact of demography and genetics. Despite no evidence for demographic rescue, I found genetic rescue in one recipient population. Divergent immigrants did not have a negative effect in almost all cases, and any positive effect they had depended on the genetic diversity, immigrant fitness, and recipient life-history traits. Together, these experiments provide strong evidence that immigrants can bolster population fitness despite being divergent, thereby supporting the use of augmentation as a management technique in dire situations when no safe immigrant sources are available.
Open Access
Population ecology of black-footed ferrets (Mustela nigripes) in relation to sylvatic plague
(Colorado State University. Libraries, 2023) Livieri, Travis M., author; Angeloni, Lisa, advisor; Antolin, Michael, committee member; Biggins, Dean, committee member; Crooks, Kevin, committee member
Infectious diseases can have significant impacts, both direct and indirect, on the conservation of endangered species. A full understanding of these impacts is hindered by the difficulty of teasing apart disease effects from other factors that led to endangerment, the scarcity of population data from before and after disease detection, and the inherent challenge of studying rare species, which are often difficult to detect. Ideally, a disease and population monitoring strategy will detect outbreaks so effective management and mitigation strategies can be implemented. Disease mitigation strategies, such as vaccination or removal of infected individuals, can be effective but costly to implement and rigorous evaluations of such efforts are rare. Here we present a case study and evaluation of a multi-faceted effort to manage multiple impacts of sylvatic plague (plague hereafter), an invasive disease, in a reintroduced population of endangered black-footed ferrets (Mustela nigripes) and their prey, black-tailed prairie dogs (Cynomys ludovicianus), in Conata Basin/Badlands National Park, South Dakota. Since reintroduction in 1994-1999, this is the largest free-ranging ferret population. Chapter One provides a broad introduction to black-footed ferret natural history, ecology, and conservation efforts. We briefly described the life history of black-footed ferrets, their reliance upon prairie dogs (Cynomys spp.) as prey and habitat engineers, and the conflicts between prairie dogs and agricultural interests that motivated human efforts to eradicate prairie dogs and inadvertently drove ferrets towards extinction. Ensuing captive breeding and reintroduction efforts averted extinction of the species, but plague, caused by Yersinia pestis bacteria, led to high mortality in both black-footed ferrets and prairie dogs, was a second factor in ferret decline, and continues to threaten reintroduced populations. Plague management, through flea vector control and vaccination, is a high priority for the black-footed ferret recovery program, along with maintaining genetic diversity and securing habitat. We concluded that black-footed ferret recovery to date has been partially successful, but challenges remain, and plague represents the largest biological threat. In Chapter Two, we evaluated the efforts to manage plague for black-footed ferrets and prairie dogs at Conata Basin/Badlands National Park. We effectively monitored plague using carnivore serology, prairie dog testing, and visual surveys to detect the invasion of plague and inform our mitigation efforts. Both prairie dog colonies and black-footed ferret populations declined precipitously with the plague epizootic. We applied deltamethrin dust into prairie dog burrows to kill fleas and vaccinated black-footed ferrets against plague during annual monitoring efforts. Our results suggested that dusting was effective in maintaining prairie dog colonies compared to non-dusted colonies and significantly increasing survival of black-footed ferrets. Additionally, our vaccination of black-footed ferrets added incremental gains in ferret survival. These combined efforts of plague surveillance, dusting prairie dog burrows, and vaccinating black-footed ferrets likely prevented extirpation of this population. In Chapter Three, we used stable isotope analysis to understand the effects of plague on the proportion of prairie dogs in black-footed ferret diets. Previous studies on black-footed ferrets found up to one-third of ferret diet is comprised of non-prairie dog rodents. Plague causes high mortality in prairie dogs and other small mammals found on prairie dog colonies, potentially increasing variability in prey available for black-footed ferrets. We sampled black-footed ferrets and two prey items, prairie dogs and deer mice (Peromyscus sonoriensis), before and during a plague epizootic and used stable isotope analysis to estimate the diet proportions in relation to plague and dusting. We found that prior to plague black-footed ferret diets in Conata Basin/Badlands National Park were similar to previous studies, but during a plague epizootic ferrets shifted their diet almost completely to prairie dogs. Dusting prairie dog burrows prior to the invasion of plague had a similar effect in shifting black-footed ferret diets. We concluded that despite observed foraging plasticity, black-footed ferrets can be considered prairie dog colony specialists, and any diet effects following deltamethrin dust treatment are likely less severe than the impacts of plague on unprotected ferret populations.
Open Access
The use of acoustic collars for studying landscape effects on animal behavior
(Colorado State University. Libraries, 2013) Lynch, Emma, author; Angeloni, Lisa, advisor; Wittemyer, George, advisor; Crooks, Kevin, committee member; Fristrup, Kurt, committee member
Audio recordings made from free-ranging animals can be used to investigate aspects of physiology, behavior, and ecology through acoustic signal processing. On-animal acoustical monitoring applications allow continuous remote data collection, and can serve to address questions across temporal and spatial scales. We report on the design of an inexpensive collar-mounted recording device and present data on the activity budget of wild mule deer (Odocoileus hemionus) derived from these devices, which were applied for a two-week period. Over 3,300 hours of acoustical recordings were collected from 10 deer on their winter range in a natural gas extraction field in northwestern Colorado. Results demonstrated that acoustical monitoring is a viable and accurate method for characterizing individual time budgets and behaviors of ungulates. This acoustical monitoring technique also provides a new approach to investigate the ways external forces affect wildlife behavior. One particularly salient activity revealed by our acoustical monitoring was periodic pausing by mule deer within bouts of mastication, which appear to be adopted for listening for environmental cues of interest. While visual forms of vigilance, such as scanning or alert behavior, have been well documented across a wide range of animal taxa, animals also employ other vigilance modalities such as auditory vigilance, by listening for the acoustic cues of predators. To better understand the ecological properties that structure this behavior, we examined how natural and anthropogenic landscape variables influenced the amount of time that mule deer paused during mastication bouts. We found that deer paused more where concealment cover abounded, and where visual vigilance was likely to be less effective. Additionally, deer paused more often at night than they did during the day, and in areas of moderate background sound levels. Our results support the idea that pauses during mastication represent a form auditory vigilance that is responsive to landscape variables. Furthermore, these results suggest that exploring this behavior is critical to understanding an animal's perception of risk and the costs associated with vigilance behavior.