Theses and Dissertations

Permanent URI for this collection

Now showing 1 - 1 of 1

Open Access
Tissue engineering of heart valves: antigen removal from xenogeneic tissue scaffolds
(Colorado State University. Libraries, 2009) Arai, Shiori, author; Orton, E. Christopher, advisor
Tissue-engineered heart valves hold the promise of an ideal heart valve substitute by using appropriate and functional cells and scaffolds. An ideal heart valve should be durable, non-immunogenic, non-thrombogenic, resistant to infection and capable of regeneration and growth. Xenogeneic tissues are potential candidates for scaffolding of tissue-engineered heart valves. Anionic detergent-based decellularization has been employed to eliminate xenogeneic tissue immunogenicity. The present studies were performed to develop a technique to detect antigenic proteins in xenogeneic tissue scaffolds, to evaluate the efficacy of antigen removal of current detergent-based decellularization of xenogeneic tissues, to develop novel techniques to enhance antigen removal, and to address issues related to the cytotoxic effects of sodium dodecyl sulfate (SDS).