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Browsing Student Projects by Author "Cappa, Jennifer J., author"

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    Phylogeny of Celastraceae (spindle-tree family) subfamilies Hippocrateoideae and Salacioideae inferred from chloroplast and nuclear genes
    (Colorado State University. Libraries, 2008) Coughenour, Jennifer Marie, author; Simmons, Mark P., author; Lombardi, Julio A., author; Archer, Robert H., author; Cappa, Jennifer J., author
    The phylogeny of Celastraceae (the spindle-tree family) subfamilies Hippocrateoideae and Salacioideae, which include about 360 species of shrubs, trees, and vines in 25 genera, was inferred using plastid (matK, trnL-F) and nuclear (ITS and 26S rDNA) genes. Together, subfamilies Hippocrateoideae and Salacioideae contain all members of the former Hippocrateaceae, which are now recognized as a derived group within Celastraceae sensu stricto. Based on our results, Brassiantha, a monotypic genus endemic to New Guinea, is more closely related to the clade of Dicarpellum (New Caledonia) and Hypsophila (Queensland, Australia) than it is to the former Hippocrateaceae, in contrast to previous studies. This well supported resolution indicates that having a nectary disk positioned outside the stamens has been convergently derived in these two separate lineages. The clade of Kokoona and Lophopetalum was resolved as sister to the clade of Hippocrateoideae, Sarawakodendron, and Salacioideae. This resolution of Kokoona and Lophopetalum supports previous assertions that they are a "transitional link" between Celastraceae sensu stricto and the former Hippocrateaceae. Sarawakodendron, a monotypic genus endemic to Borneo, was resolved as sister to the clade of Salacioideae, which supports earlier assertions that Sarawakodendron is "transitional" between Kokoona, Lophopetalum, and Salacioideae. Based on our inferred phylogeny, arils as mucilaginous pulp are derived within Salacioideae and winged arils may be primitive within the former Hippocrateaceae as a whole. Finally, the former Hippocrateaceae had an Old World, rather than a New World, origin.
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    Phylogeny of the Celastreae (Celastraceae) inferred using chloroplast and nuclear loci
    (Colorado State University. Libraries, 2007) Cappa, Jennifer J., author; Daw, Allison L., author; Simmons, Mark P., author
    The phylogenetic inference presented of the Celastreae (Celastraceae) is based on four loci: matK and trnL-F from the chloroplast genome and ITS and 26S rDNA from the nuclear genome. The species sampled are combined with taxa sampled from previous studies to better test the monophyly of the genera along with the intergeneric relationships within this tribe. The main purpose is to give an overview of the laboratory procedures used and the results from the sampling. The procedures include DNA isolation, DNA amplification, DNA purification, construction of contiguous sequences, sequence alignment, and lastly phylogenetic inference.