Effect of Agrobacterium co-cultivation time on recovery of transgenic Petunia hybrida plants
Agrobacterium-mediated transformation genetically alters plant cells by transferring a DNA fragment (T-DNA) from the bacterial cell to the host species. This method is useful to insert traits of interest in plants for research and commercial purposes. Agrobacterium regrowth and subsequent culture contamination is a common problem leading to culture necrosis and poor recovery of transgenic plants. The goal of this research was to study the effect of Agrobacterium co-cultivation time on the recovery of transgenic cultures and plants in Petunia hybrida cultivar Mitcham. Petunia seeds were ...
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