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dc.contributor.advisorGatlin, Jesse
dc.contributor.authorZumo, Jacob
dc.contributor.authorZumo, Jacob
dc.date2017-05-13
dc.date.accessioned2018-06-10T21:36:05Z
dc.date.available2018-06-10T21:36:05Z
dc.description.abstractMitotic spindles play a key role in cellular division. These structures, which are composed of dynamic filaments called microtubules, are responsible for separation and segregation of chromosomes during mitosis. Spindles must be the correct shape and size to insure fidelity of this process, however, their formation and assembly are still not entirely understood. For example, the mechanisms that govern spindle shape and determine individual spindle size for a given cell type are still unknown. Based on evidence from recent studies of spindle scaling, in which spindle size effectively scaled with cell size, we hypothesize that within small cytoplasmic volumes, spindle building blocks become limiting and thereby limit spindle size. This hypothesis predicts that microtubule dynamics within the spindle will be adversely affected by changes in cytoplasmic volume. By combining cell-free cytoplasmic extracts, microfluidics, and confocal microscopy, we hope to measure changes in microtubule dynamics to elucidate the relationship between cell volume and spindle size within the cell.
dc.identifier.urihttps://hdl.handle.net/20.500.11919/1426
dc.languageEnglish
dc.publisherUniversity of Wyoming. Libraries
dc.relation.ispartofHonors Theses AY 16/17
dc.relation.ispartofHonors Theses AY 16/17
dc.sourceHonors Theses AY 16/17
dc.subjectmicrotubules
dc.subjectmicrotubule dynamics
dc.subjectspindles
dc.subjectspindle assembly
dc.subjectcytoplasmic volume
dc.titleLimiting Effect of Cytoplasmic Volume on Microtubule Dynamics, The
dc.typeThesis
thesis.degree.disciplineMolecular Biology


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