Investigating the role of mir-190, mir-200, and mir-8 inhibition of the GCS protein in HeLa cells and Drosophila melanogaster
Glucosylceramide synthase (GCS or GlcT-1 in Drosophila and UGCG in humans) is a key enzyme in sphingolipid metabolism and obesity. Pharmacological inhibition of GCS in obese mice improves their insulin responsiveness, adipocyte functions, and also reduces inflammation and liver fat content. MicroRNAs are non-coding RNAs that specifically inhibit genes by binding to target sites in the gene's mRNA. Based on preliminary evidence that GCS is regulated by microRNAs in vitro and that it contains well conserved binding sites for the microRNAs mir-8, mir-190, and mir-200, we propose to investigate the ...
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