Analysis of Sugars and Amino Acids in Aphid Honeydew by Hydrophilic Interaction Liquid Chromatography-Mass Spectrometry
The impact of phenology to herbivore abundance was studied on the subalpine plant Ligusticum porteri and the aphid herbivore Aphis asclepiadis to assess some of the impact of climate change on this herbivore-plant system. The honeydew production of A. asclepiadis feeding on L. porteri under different phenological mismatches was examined in terms of sugar and amino acid composition. Given the difficulties of analyzing sugars and amino acids, a recent alternative separation mode for HPLC, hydrophilic interaction liquid chromatography (HILIC), was employed. As HILIC separation mechanisms are based ...
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