Marconi, Mario Carlo, authorEtcheverry, J. I., authorTorga, J. R., authorElsevier Science B. V., publisher2007-01-032007-01-031997Torga, J. R., J. I. Etcheverry, and M. C. Marconi, Design of a Fluorescence Technique Using Double Laser Pulse Excitation for the Measurement of Molecular Brownian Dynamics, Optics Communications 143, no. 4-6 (15 November 1997): [230]-234.http://hdl.handle.net/10217/67850A complete analysis of a novel method to evaluate the rotational diffusion time of fluorescent molecules is presented. It is based on the study of the total fluorescence produced after excitation with two consecutive short laser pulses. Expressions for the total fluorescence energy emitted in this case are derived, which would allow the evaluation of the rotational diffusion time. The main advantages of the proposed method are its simple setup, a time resolution only limited by the exciting laser pulsewidth, and the fact that it does not require fast time response detectors.born digitalarticleseng©1997 Elsevier Science B. V.Copyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.Brownian dynamicsfluorescence anisotropyrotational depolarizationText