Carranza, Kasimir, authorAllen, Christopher P., authorHenao-Tamayo, Marcela, authorHanneman, William, author2017-11-132017-11-132017https://hdl.handle.net/10217/184864Pumping breast milk and freezing it is common practice in hospitals, neonatal intensive care units (NICU), and numerous households. Freezing milk may alter the cellular constituency of breast milk by reducing the amount of stem cells. Here, we investigate the alteration of stem cell viability and concentration in human breast milk frozen at varying temperatures and durations. This reduction may negatively impact infant development; vulnerable populations such as preterm infants have higher nutritional requirements and less volumetric capacity in their stomach. Freezing milk may undermine attempts to supply preterm infants with the nutritional requirements needed to sustain life.born digitalStudent workspostersengCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.stem cellsflow cytometrypreterm infantshuman breast milkText