Preliminary Characterization of Hia, an Auto-secreting Protein of Brucella abortus, A
Hia, a Type-V auto-secretion protein has been suggested as a possible marker for detecting brucellosis in cattle using a lateral flow-based assay system. Several studies have suggested that Hia may be important for the pathogenesis of brucellosis infection, so this project investigated various physical properties of the protein. Gel filtration chromatography and polyacrylamide electrophoresis gel suggest that the Hia monomer is a protein approximately 66-68 kDa in size. This study found that, while having the ability to form much larger aggregates, Hia preferentially forms dimers in a native ...
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