Spectrally resolved fluorescence microscopy and its applications for single molecule characterization
Spectrally resolved fluorescence microscopy is an important tool for measuring biological systems at the single molecule level. Spectral resolution provides a means for unambiguous identification and a tool for characterizing heterogeneity. Fluorescence microscopy facilitates single molecule measurement by limiting contrast to only those molecules that are capable of producing fluorescence when illuminated with the excitation source of the system. In the following thesis I describe my work combining spectral resolution with a fluorescence microscope by developing a hyperspectral prism-type total ...