Development of asymmetrical flow field-flow fractionation for the characterization of proteins, protein aggregation, and nanoparticles
Field-flow fractionation (FFF) is a family of analytical techniques used to characterize macromolecules and particles from ~1 nm to >1µm. Its versatility has allowed a number of analytical challenges to be addressed, but several limitations still exist. Advances in asymmetrical flow field-flow fractionation (AF4) coupled with UV-Vis, multiangle light scattering (MALS), and/or dynamic light scattering (DLS) were made to overcome current limitations in the characterization of proteins, protein aggregates, and nanoparticles. Formation of protein aggregates in protein therapeutics is a major concern ...
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