Anti-CD8 monoclonal antibody-mediated depletion alters the phenotype and functionality of surviving CD8+ T cells
It is common practice for researchers to use antibodies to remove a specific cell type to infer its function. However, it is difficult to completely eliminate a cell type and there is often limited or no information as to how the cells which survive depletion are effected. This is particularly important for CD8+ T cells for two reasons. First, they are more resistant to mAb-mediated depletion than other lymphocytes. Second, targeting either the CD8α or CD8β chain could induce differential effects. I show here that administration of two commonly used depleting-mAbs, targeting either the CD8α or ...
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