Now showing items 1-9 of 9
Genome-wide mapping of the DNA modification uracil and its mechanism of incorporation into DNA
Author(s):Bryan, Debra Suzi
The incorporation and creation of modified nucleobases in DNA have profound effects on genome function. In this study, we developed a novel, specific method for mapping positions and local content of modified DNA nucleobases ...
Thyroid hormone responsive protein Spot14 modulates fatty acid synthase kinetics and fatty acid products
Author(s):Rudolph, Michael C.
The mammary gland synthesizes and secretes a complex fluid, milk, that is balanced in nutrient content and uniquely tailored to the growth and development of the neonate. Milk is comprised of three primary nutrients ...
Cellular receptors in cancer: CD147 and Mer
The identification of oncogenic cellular receptors and the characterization of their underlying molecular mechanisms is essential to our understanding of cancer progression and our approach to treating malignancies. The ...
Chromatin dynamics and genomic instability during replicative aging
Histones are the fundamental protein subunit of chromatin and have a profound impact on processes such as chromatin assembly/disassembly and diseases such as aging. I examined the functional importance of mutating the ...
Molecular analysis of RNA polymerase ll elongation and termination on mammalian genes
Author(s):Brannan, Kristopher Wade
RNA polymerase II (pol II) pausing, elongation and termination are important mechanisms for controlling pol II distribution and transcriptional output during the transcription cycle on protein coding genes. This thesis ...
Citrullinated fibrinogen-specific CD4 T cell lines in mouse models of rheumatoid arthritis
Author(s):Cordova, Kristen Nordick
Citrullinated proteins, derived from the conversion of peptidyl-arginine to peptidyl-citrulline, are present in the joints of patients with rheumatoid arthritis (RA), who also uniquely produce high levels of anti-citrullinated ...
Apical protein transport: the role of FIP5 in epithelial lumen morphogenesis
Author(s):Willenborg, Carly Michelle
During the morphogenesis of the epithelial lumen, apical proteins are transported via endocytic compartments to the site of the forming lumen, although the machinery mediating this transport remains to be elucidated. Rab11 ...
Tale of two IRESes: translational regulation mediated by the eukaryotic FMR1 and Aurora A IRESes in disease, A
Author(s):Dobson, Tara Heather Weiland
Regulation of translation initiation of most eukaryotic mRNAs occurs in a cap-dependent manner. In this study I demonstrate how misregulated translation of two eukaryotic mRNAs via internal ribosomal entry sites (IRESes) ...
Biophysical and functional studies of pRNA: a self-associating RNA
The packaging RNA (pRNA) found in the phi29 family of bacteriophage is an essential component of a powerful molecular motor used to package the phage's DNA genome into the capsid. The pRNA forms a higher-order multimer by ...