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    Genome-wide mapping of the DNA modification uracil and its mechanism of incorporation into DNA

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    http://hdl.handle.net/10968/1142
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    Genome-wide mapping of the DNA modification uracil and its mechanism of incorporation into DNA (17.91Mb)
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    Abstract
    The incorporation and creation of modified nucleobases in DNA have profound effects on genome function. In this study, we developed a novel, specific method for mapping positions and local content of modified DNA nucleobases through combination of in vitro base excision with massively parallel DNA sequencing. We applied this method, named Excision-seq, to map uracil in Escherichia coli and Saccharomyces cerevisiae, and discovered significant variation in uracil content wherein uracil is excluded from the earliest and latest-replicating regions of the genome. Widely used antimetabolite chemotherapies ...
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    Author(s)
    Bryan, Debra Suzi

    Advisor(s)
    Hesselberth, Jay R.; Johnston, Mark

    Rights Access
    Embargo Expires: 08/19/2016

    Date Submitted
    2015
    Collections
    • 2012 - 2016
    • Theses and Dissertations - School of Medicine

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