Genome-wide mapping of the DNA modification uracil and its mechanism of incorporation into DNA
The incorporation and creation of modified nucleobases in DNA have profound effects on genome function. In this study, we developed a novel, specific method for mapping positions and local content of modified DNA nucleobases through combination of in vitro base excision with massively parallel DNA sequencing. We applied this method, named Excision-seq, to map uracil in Escherichia coli and Saccharomyces cerevisiae, and discovered significant variation in uracil content wherein uracil is excluded from the earliest and latest-replicating regions of the genome. Widely used antimetabolite chemotherapies ...
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