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dc.contributor.advisorMcNaughton, Brian
dc.contributor.authorDePorter, Sandra M.
dc.contributor.committeememberKennan, Alan
dc.contributor.committeememberCrans, Debbie
dc.contributor.committeememberReynolds, Melissa
dc.contributor.committeememberDi Pietro, Santiago
dc.date.accessioned2007-01-03T05:57:16Z
dc.date.available2017-01-31T06:30:24Z
dc.date.issued2014
dc.description2014 Fall.
dc.descriptionIncludes bibliographical references.
dc.description.abstractProteinaceous reagents, including antibodies and synthetic proteins, have become some of the most effective reagents for targeted treatment and diagnosis of disease. The unique catalytic activity of some proteins and ability to bind disease-relevant receptors that can evade small molecule discovery, make these reagents well suited for use as therapeutic and bioimaging reagents. However, the large size and charge distribution of most proteins greatly inhibits their intracellular delivery to diseased cells, limiting targets to those displayed on the cell surface. In response to this challenge, we have developed a bacteriophage nanocarrier to deliver large payloads of proteinaceous cargo to the interior of prostate cancer cells. This reagent employs two distinct components: a genetically defined prostate cancer cell-selective protein transduction domain, and a biotinylation site on an orthogonal coat protein, which allows for complexation with streptavidin fusion proteins. Collectively, this approach permits targeted intracellular delivery of ~20 exogenous proteins per phage to human prostate cancer cells. This multifunctional technology offers a cell-selective solution to the challenges associated with delivering protein cargo to the interior of diseased cells and may lead to an expansion in the use of protein reagents.
dc.format.mediumborn digital
dc.format.mediumdoctoral dissertations
dc.identifierDePorter_colostate_0053A_12661.pdf
dc.identifier.urihttp://hdl.handle.net/10217/88422
dc.languageEnglish
dc.publisherColorado State University. Libraries
dc.relation.ispartof2000-2019 - CSU Theses and Dissertations
dc.rightsCopyright of the original work is retained by the author.
dc.titleEngineering bacteriophage nanocarriers for targeted delivery of protein reagents to prostate cancer cells
dc.typeText
dcterms.embargo.expires2017-01-31
dcterms.rights.dplaThe copyright and related rights status of this Item has not been evaluated (https://rightsstatements.org/vocab/CNE/1.0/). Please refer to the organization that has made the Item available for more information.
thesis.degree.disciplineChemistry
thesis.degree.grantorColorado State University
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Philosophy (Ph.D.)


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