Factor dependent archaeal transcription termination
RNA polymerase activity is regulated by nascent RNA sequences, DNA template sequences and conserved transcription factors. Transcription factors regulate the activities of RNA polymerase (RNAP) at each stage of the transcription cycle: initiation, elongation, and termination. Many basal transcription factors with common ancestry are employed in eukaryotic and archaeal systems that directly bind to RNAP and influence intramolecular movements of RNAP and modulate DNA or RNA interactions. We describe and employ a flexible methodology to directly probe and quantify the binding of transcription factors ...
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