Fluorescent labeling of extracellular markers on stem cells from human breast milk
Date
2017
Authors
Carranza, Kasimir, author
Allen, Christopher P., author
Henao-Tamayo, Marcela, author
Hanneman, William, author
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Abstract
Pumping breast milk and freezing it is common practice in hospitals, neonatal intensive care units (NICU), and numerous households. Freezing milk may alter the cellular constituency of breast milk by reducing the amount of stem cells. Here, we investigate the alteration of stem cell viability and concentration in human breast milk frozen at varying temperatures and durations. This reduction may negatively impact infant development; vulnerable populations such as preterm infants have higher nutritional requirements and less volumetric capacity in their stomach. Freezing milk may undermine attempts to supply preterm infants with the nutritional requirements needed to sustain life.
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Subject
stem cells
flow cytometry
preterm infants
human breast milk