Open Access Research and Scholarship Fund (OARS)

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Since 2013, the Colorado State University Libraries, under the guidance of the CSU Faculty Council Committee on Libraries, has allocated funds to support the Open Access Research and Scholarship (OARS) Fund. The fund is open to CSU tenured/tenure track faculty and full-time research scientists. This digital collection contains articles that received OARS funding between 2011 and 2020.

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Open Access
Accurate characterization of winter precipitation using multi-angle snowflake camera, visual hull, advanced scattering methods and polarimetric radar
(Colorado State University. Libraries, 2016-06-11) Notaroš, Branislav M., author; Bringi, Viswanathan N., author; Kleinkort, Cameron, author; Kennedy, Patrick, author; Huang, Gwo-Jong, author; Thurai, Merhala, author; Newman, Andrew J., author; Bang, Wonbae, author; Lee, GyuWon, author; MDPI, publisher
This article proposes and presents a novel approach to the characterization of winter precipitation and modeling of radar observables through a synergistic use of advanced optical disdrometers for microphysical and geometrical measurements of ice and snow particles (in particular, a multi-angle snowflake camera-MASC), image processing methodology, advanced method-of-moments scattering computations, and state-of-the-art polarimetric radars. The article also describes the newly built and established MASCRAD (MASC + Radar) in-situ measurement site, under the umbrella of CSU-CHILL Radar, as well as the MASCRAD project and 2014/2015 winter campaign. We apply a visual hull method to reconstruct 3D shapes of ice particles based on high-resolution MASC images, and perform "particle-by-particle" scattering computations to obtain polarimetric radar observables. The article also presents and discusses selected illustrative observation data, results, and analyses for three cases with widely-differing meteorological settings that involve contrasting hydrometeor forms. Illustrative results of scattering calculations based on MASC images captured during these events, in comparison with radar data, as well as selected comparative studies of snow habits from MASC, 2D video-disdrometer, and CHILL radar data, are presented, along with the analysis of microphysical characteristics of particles. In the longer term, this work has potential to significantly improve the radar-based quantitative winter-precipitation estimation.
Open Access
Age-related changes in arterial blood-gas variables in Holstein calves at moderate altitude
(Colorado State University. Libraries, 2014-05-15) Neary, Joseph M., author; Garry, Franklyn B., author; Raabis, Sarah M., author; Dove Medical Press Ltd., publisher
The goal of this study was to determine whether peripheral oxygen delivery and efficacy of alveolar-arterial oxygen (A-a O2) transfer, as estimated from the A-a O2 pressure gradient, are compromised in Holstein calves at moderate altitude. The primary objective was to evaluate age-related changes in arterial blood-gas variables, L-lactate, and hematocrit in healthy calves. The secondary objective was to determine if coughing and nasal discharge, commonly used indicators of respiratory disease, are associated with A-a O2 gradient. Arterial blood-gas tensions were evaluated in a cohort of 61 dairy calves on one farm at moderate altitude (1,601 m to 1,696 m). Sampling was performed on four occasions at approximately 10, 38, 150, and 261 days of age. Hyperventilation, as indicated by hypocapnia, was evident in calves of all ages. Increasing age was associated with a nonlinear increase in arterial oxygen tension (P<0.001) and a nonlinear decrease in A-a O2 gradient (P<0.001). The mean A-a O2 gradient at 10 and 38 days of age was over 18 mmHg, indicating poor efficacy of oxygen transfer. Cough score (P=0.02) but not nasal score (P=0.32) was associated with an in increase in A-a O2 pressure gradient. Mean hematocrit remained low (<27%) despite hypoxemia. From 38 days of age, median L-lactate concentration remained over 1.5 mmol/L, indicating substantial anaerobic respiration due to inadequate oxygen delivery. Twenty-five percent of calves were treated for respiratory disease. The maximum age at first treatment was 102 days. In conclusion, there was a nonlinear improvement in A-a O2 transfer efficacy with increasing age, but peripheral oxygen delivery remained compromised. Hyperventilation and impaired A-a O2 transfer due to functional immaturity of the pulmonary system may be risk factors for respiratory disease in dairy calves at moderate altitude.
Open Access
Arabidopsis thaliana VOZ (Vascular plant One-Zinc finger) transcription factors are required for proper regulation of flowering time
(Colorado State University. Libraries, 2013-04-15) Celesnik, Helena, author; Ali, Gul S., author; Robison, Faith M., author; Reddy, Anireddy S. N., author; The Company of Biologists Ltd., publisher
Transition to flowering in plants is tightly controlled by environmental cues, which regulate the photoperiod and vernalization pathways, and endogenous signals, which mediate the autonomous and gibberellin pathways. In this work, we investigated the role of two Zn2+-finger transcription factors, the paralogues AtVOZ1 and AtVOZ2, in Arabidopsis thaliana flowering. Single atvoz1-1 and atvoz2-1 mutants showed no significant phenotypes as compared to wild type. However, atvoz1-1 atvoz2-1 double mutant plants exhibited several phenotypes characteristic of flowering-time mutants. The double mutant displayed a severe delay in flowering, together with additional pleiotropic phenotypes. Late flowering correlated with elevated expression of FLOWERING LOCUS C (FLC), which encodes a potent floral repressor, and decreased expression of its target, the floral promoter FD. Vernalization rescued delayed flowering of atvoz1-1 atvoz2-1 and reversed elevated FLC levels. Accumulation of FLC transcripts in atvoz1-1 atvoz2-1 correlated with increased expression of several FLC activators, including components of the PAF1 and SWR1 chromatin-modifying complexes. Additionally, AtVOZs were shown to bind the promoter of MOS3/SAR3 and directly regulate expression of this nuclear pore protein, which is known to participate in the regulation of flowering time, suggesting that AtVOZs exert at least some of their flowering regulation by influencing the nuclear pore function. Complementation of atvoz1-1 atvoz2-1 with AtVOZ2 reversed all double mutant phenotypes, confirming that the observed morphological and molecular changes arise from the absence of functional AtVOZ proteins, and validating the functional redundancy between AtVOZ1 and AtVOZ2.
Open Access
Behavioral responses associated with a human-mediated predator shelter
(Colorado State University. Libraries, 2014-04) Graeme, Shannon, author; Cordes, Line S., author; Hardy, Amanda R., author; Angeloni, Lisa M., author; Crooks, Kevin R., author; Public Library of Science, publisher
Human activities in protected areas can affect wildlife populations in a similar manner to predation risk, causing increases in movement and vigilance, shifts in habitat use and changes in group size. Nevertheless, recent evidence indicates that in certain situations ungulate species may actually utilize areas associated with higher levels of human presence as a potential refuge from disturbance-sensitive predators. We now use four-years of behavioral activity budget data collected from pronghorn (Antilocapra americana) and elk (Cervus elephus) in Grand Teton National Park, USA to test whether predictable patterns of human presence can provide a shelter from predatory risk. Daily behavioral scans were conducted along two parallel sections of road that differed in traffic volume - with the main Teton Park Road experiencing vehicle use that was approximately thirty-fold greater than the River Road. At the busier Teton Park Road, both species of ungulate engaged in higher levels of feeding (27% increase in the proportion of pronghorn feeding and 21% increase for elk), lower levels of alert behavior (18% decrease for pronghorn and 9% decrease for elk) and formed smaller groups. These responses are commonly associated with reduced predatory threat. Pronghorn also exhibited a 30% increase in the proportion of individuals moving at the River Road as would be expected under greater exposure to predation risk. Our findings concur with the 'predator shelter hypothesis', suggesting that ungulates in GTNP use human presence as a potential refuge from predation risk, adjusting their behavior accordingly. Human activity has the potential to alter predator-prey interactions and drive trophic-mediated effects that could ultimately impact ecosystem function and biodiversity.
Open Access
BIM use by architecture, engineering, and construction (AEC) industry in educational facility projects
(Colorado State University. Libraries, 2019-07-03) Moreno, Cristina, author; Olbina, Svetlana, author; Issa, Raja R., author; Hindawi, publisher
In recent years, many public and private sector owners have started to require a building information modeling (BIM) component in new construction projects. Although there has been a significant increase in industry-wide acceptance of BIM, it is still not a standard practice in the educational facility sector. This research aimed at exploring the use of BIM in educational facility projects by the architecture, engineering, and construction (AEC) disciplines. A survey that investigated BIM adoption at the company level, BIM implementation in projects, benefits of using BIM, and obstacles to using BIM was distributed to architects, site engineers, structural engineers, mechanical engineers, and contractors across the United States. The survey results showed that a majority of the respondents from all five disciplines used BIM. BIM was most commonly used for 3D visualization, automation of documentation, and clash detection. The most important benefits of BIM included better marketing and clearer understanding of projects which is crucial for clients such as school students, teachers, and principals. Lack of expertise and need for training seemed to be main obstacles to BIM use. The research contributes to the body of knowledge by showing prevalence of BIM use on educational facility projects and indicating how BIM could help improve collaborative knowledge sharing among designers, contractors, and clients, resulting in better quality educational buildings. These research findings can be used to assist AEC companies that are interested in implementing BIM in the educational facility projects.
Open Access
Biomass for thermochemical conversion: targets and challenges
(Colorado State University. Libraries, 2013-07) Tanger, Paul, author; Field, John L., author; Jahn, Courtney E., author; DeFoort, Morgan W., author; Leach, Jan E., author; Frontiers Research Foundation, publisher
Bioenergy will be one component of a suite of alternatives to fossil fuels. Effective conversion of biomass to energy will require the careful pairing of advanced conversion technologies with biomass feedstocks optimized for the purpose. Lignocellulosic biomass can be converted to useful energy products via two distinct pathways: enzymatic or thermochemical conversion. The thermochemical pathways are reviewed and potential biotechnology or breeding targets to improve feedstocks for pyrolysis, gasification, and combustion are identified. Biomass traits influencing the effectiveness of the thermochemical process (cell wall composition, mineral and moisture content) differ from those important for enzymatic conversion and so properties are discussed in the language of biologists (biochemical analysis) as well as that of engineers (proximate and ultimate analysis). We discuss the genetic control, potential environmental influence, and consequences of modification of these traits. Improving feedstocks for thermochemical conversion can be accomplished by the optimization of lignin levels, and the reduction of ash and moisture content. We suggest that ultimate analysis and associated properties such as H:C, O:C, and heating value might be more amenable than traditional biochemical analysis to the high-throughput necessary for the phenotyping of large plant populations. Expanding our knowledge of these biomass traits will play a critical role in the utilization of biomass for energy production globally, and add to our understanding of how plants tailor their composition with their environment.
Open Access
Building a multi-institutional and interdisciplinary team to develop a zoonotic tuberculosis roadmap
(Colorado State University. Libraries, 2018-06-12) Olea-Popelka, Francisco, author; Fujiwara, Paula I., author; Frontiers in Public Health, publisher
Tuberculosis (TB), as the major infectious disease in the world, has devastating consequences for not only humans, but also cattle and several wildlife species. This disease presents additional challenges to human and veterinary health authorities given the zoonotic nature of the pathogens responsible for the disease across species. One of the main public health challenges regarding zoonotic TB (ZTB) caused by Mycobacterium bovis is that the true incidence of this type of TB in humans is not known and is likely to be underestimated. To effectively address challenges posed by ZTB, an integrated One Health approach is needed. In this manuscript, we describe the rationale, major steps, timeline, stakeholders, and important events that led to the assembling of a true integrated multi-institutional and interdisciplinary team that accomplished the ambitious goal of developing a ZTB roadmap, published in October, 2017. It outlines key activities to address the global challenges regarding the prevention, surveillance, diagnosis, and treatment of ZTB. We discuss and emphasize the importance of integrated approaches to be able to accomplish the short (year 2020) and medium term (year 2025) goals outlined in the ZTB roadmap.
Open Access
Carbon-degrading enzyme activities stimulated by increased nutrient availability in Arctic tundra soils
(Colorado State University. Libraries, 2013-10) Koyama, Akihiro, author; Wallenstein, Matthw D., author; Simpson, Rodney T., author; Moore, John C., author; Public Library of Science, publisher
Climate-induced warming of the Arctic tundra is expected to increase nutrient availability to soil microbes, which in turn may accelerate soil organic matter (SOM) decomposition. We increased nutrient availability via fertilization to investigate the microbial response via soil enzyme activities. Specifically, we measured potential activities of seven enzymes at four temperatures in three soil profiles (organic, organic/mineral interface, and mineral) from untreated native soils and from soils which had been fertilized with nitrogen (N) and phosphorus (P) since 1989 (23 years) and 2006 (six years). Fertilized plots within the 1989 site received annual additions of 10 g N⋅m-2⋅year-1 and 5 g P⋅m-2⋅year-1. Within the 2006 site, two fertilizer regimes were established - one in which plots received 5 g N⋅m-2⋅year-1 and 2.5 g P⋅m-2⋅year-1 and one in which plots received 10 g N⋅m-2⋅year-1 and 5 g P⋅m-2⋅year-1. The fertilization treatments increased activities of enzymes hydrolyzing carbon (C)-rich compounds but decreased phosphatase activities, especially in the organic soils. Activities of two enzymes that degrade N-rich compounds were not affected by the fertilization treatments. The fertilization treatments increased ratios of enzyme activities degrading C-rich compounds to those for N-rich compounds or phosphate, which could lead to changes in SOM chemistry over the long term and to losses of soil C. Accelerated SOM decomposition caused by increased nutrient availability could significantly offset predicted increased C fixation via stimulated net primary productivity in Arctic tundra ecosystems.
Open Access
Characterising the impacts of emerging energy development on wildlife, with an eye towards mitigation
(Colorado State University. Libraries, 2012) Northrup, Joseph M., author; Wittemyer, George, author; Blackwell Publishing Ltd., publisher
Global demand for energy is projected to increase by 40% in the next 20 years, and largely will be met with alternative and unconventional sources. Development of these resources causes novel disturbances that strongly impact terrestrial ecosystems and wildlife. To effectively position ecologists to address this prevalent conservation challenge, we reviewed the literature on the ecological ramifications of this dominant driver of global land-use change, consolidated results for its mitigation and highlighted knowledge gaps. Impacts varied widely, underscoring the importance of area and species-specific studies. The most commonly reported impacts included behavioural responses and direct mortality. Examinations of mitigation were limited, but common easements included (1) reduction of the development footprint and human activity, (2) maintenance of undeveloped, 'refuge' habitat and (3) alteration of activity during sensitive periods. Problematically, the literature was primarily retrospective, focused on few species, countries, and ecoregions, and fraught with generalisations from weak inference. We advocate future studies take a comprehensive approach incorporating a mechanistic understanding of the interplay between development caused impacts and species ecology that will enable effective mitigation. Key areas for future research vital to securing a sustainable energy future in the face of development-related global change are outlined.
Open Access
Chemical and mechanical defenses vary among maternal lines and leaf ages in Verbascum thapsus L. (Scrophulariaceae) and reduce palatability to a generalist insect
(Colorado State University. Libraries, 2014-08) Alba, Christina, author; Bowers, M. Deane, author; Blumenthal, Dana, author; Hufbauer, Ruth A., author; Public Library of Science, publisher
Intra-specific variation in host-plant quality affects herbivore foraging decisions and, in turn, herbivore foraging decisions mediate plant fitness. In particular, variation in defenses against herbivores, both among and within plants, shapes herbivore behavior. If variation in defenses is genetically based, it can respond to natural selection by herbivores. We quantified intra-specific variation in iridoid glycosides, trichome length, and leaf strength in common mullein (Verbascum thapsus L, Scrophulariaceae) among maternal lines within a population and among leaves within plants, and related this variation to feeding preferences of a generalist herbivore, Trichopulsia ni Hübner. We found significant variation in all three defenses among maternal lines, with T. ni preferring plants with lower investment in chemical, but not mechanical, defense. Within plants, old leaves had lower levels of all defenses than young leaves, and were strongly preferred by T. ni. Caterpillars also preferred leaves with trichomes removed to leaves with trichomes intact. Differences among maternal lines indicate that phenotypic variation in defenses likely has a genetic basis. Furthermore, these results reveal that the feeding behaviors of T. ni map onto variation in plant defense in a predictable way. This work highlights the importance of variation in host-plant quality in driving interactions between plants and their herbivores.
Open Access
Collaborative concession in food movement networks: the uneven relations of resource mobilization
(Colorado State University. Libraries, 2019-05-21) Sbicca, Joshua, author; Luxton, India, author; Hale, James, author; Roeser, Kassandra, author; MDPI AG, Basel, Switzerland, publisher
How do food movements prioritize and work to accomplish their varied and often conflicting social change goals at the city scale? Our study investigates the Denver food movement with a mixed methods social network analysis to understand how organizations navigate differences in power and influence vis-à-vis resource exchange. We refer to this uneven process with the analytical concept of "collaborative concession". The strategic resource mobilization of money, land, and labor operates through certain collaborative niches, which constitute the priorities of the movement. Among these are poverty alleviation and local food production, which are facilitated by powerful development, education, and health organizations. Therefore, food movement networks do not offer organizations equal opportunity to carry out their priorities. Concession suggests that organizations need to lose something to gain something. Paradoxically, collaboration can produce a resource gain. Our findings provide new insights into the uneven process by which food movement organizations-and city-wide food movements overall-mobilize.
Open Access
Comparative demography of an at-risk African elephant population
(Colorado State University. Libraries, 2013) Wittemyer, George, author; Daballen, David, author; Douglas-Hamilton, Iain, author; Public Library of Science, publisher
Knowledge of population processes across various ecological and management settings offers important insights for species conservation and life history. In regard to its ecological role, charisma and threats from human impacts, African elephants are of high conservation concern and, as a result, are the focus of numerous studies across various contexts. Here, demographic data from an individually based study of 934 African elephants in Samburu, Kenya were summarized, providing detailed inspection of the population processes experienced by the population over a fourteen year period (including the repercussions of recent increases in illegal killing). These data were compared with those from populations inhabiting a spectrum of xeric to mesic ecosystems with variable human impacts. In relation to variability in climate and human impacts (causing up to 50% of recorded deaths among adults), annual mortality in Samburu fluctuated between 1 and 14% and, unrelatedly, natality between 2 and 14% driving annual population increases and decreases. Survivorship in Samburu was significantly lower than other populations with age-specific data even during periods of low illegal killing by humans, resulting in relatively low life expectancy of males (18.9 years) and females (21.8 years). Fecundity (primiparous age and inter-calf interval) were similar to those reported in other human impacted or recovering populations, and significantly greater than that of comparable stable populations. This suggests reproductive effort of African savanna elephants increases in relation to increased mortality (and resulting ecological ramifications) as predicted by life history theory. Further comparison across populations indicated that elongated inter-calf intervals and older ages of reproductive onset were related to age structure and density, and likely influenced by ecological conditions. This study provides detailed empirical data on elephant population dynamics strongly influenced by human impacts (laying the foundation for modeling approaches), supporting predictions of evolutionary theory regarding demographic responses to ecological processes.
Open Access
Confounding factors in algal phosphorus limitation experiments
(Colorado State University. Libraries, 2018-10-18) Beck, Whitney S., author; Hall, Ed K., author; PLOS ONE, publisher
Assessing algal nutrient limitation is critical for understanding the interaction of primary production and nutrient cycling in streams, and nutrient diffusing substrate (NDS) experiments are often used to determine limiting nutrients such as nitrogen (N) and phosphorus (P). Unexpectedly, many experiments have also shown decreased algal biomass on NDS P treatments compared to controls. To address whether inhibition of algal growth results from direct P toxicity, NDS preparation artifacts, or environmental covariates, we first quantified the frequency of nutrient inhibition in published experiments. We also conducted a meta-analysis to determine whether heterotrophic microbial competition or selective grazing could explain decreases in algal biomass with P additions. We then deployed field experiments to determine whether P-inhibition of algal growth could be explained by P toxicity, differences in phosphate cation (K vs. Na), differences in phosphate form (monobasic vs. dibasic), or production of H2O2 during NDS preparation. We found significant inhibition of algal growth in 12.9% of published NDS P experiments as compared to 4.7% and 3.6% of N and NP experiments. The meta-analysis linear models did not show enhanced heterotrophy on NDS P treatments or selective grazing of P-rich algae. Our field experiments did not show inhibition of autotrophic growth with P additions, but we found significantly lower gross primary productivity (GPP) and biomass-specific GPP of benthic algae on monobasic phosphate salts as compared to dibasic phosphate salts, likely because of reduced pH levels. Additionally, we note that past field experiments and meta-analyses support the plausibility of direct P toxicity or phosphate form (monobasic vs. dibasic) leading to inhibition of algal growth, particularly when other resources such as N or light are limiting. Given that multiple mechanisms may be acting simultaneously, we recommend practical, cost-effective steps to minimize the potential for P- inhibition of algal growth as an artifact of NDS experimental design.
Open Access
Devastating decline of forest elephants in Central Africa
(Colorado State University. Libraries, 2013) Maisels, Fiona, author; Strindberg, Samantha, author; Blake, Stephen, author; Wittemyer, George, author; Hart, John, author; Williamson, Elizabeth A., author; Aba'a, Rostand, author; Abitsi, Gaspard, author; Ambahe, Ruffin D., author; Amsini, Fidèl, author; Bakabana, Parfait C., author; Hicks, Thurston Cleveland, author; Bayogo, Rosine E., author; Bechem, Martha, author; Beyers, Rene L., author; Bezangoye, Anicet N., author; Boundja, Patrick, author; Bout, Nicolas, author; Akou, Marc Ella, author; Bene, Lambert Bene, author; Fosso, Bernard, author; Greengrass, Elizabeth, author; Grossmann, Falk, author; Ikamba-Nkulu, Clement, author; Ilambu, Omari, author; Inogwabini, Bila-Isia, author; Iyenguet, Fortune, author; Kiminou, Franck, author; Kokangoye, Max, author; Kujirakwinja, Deo, author; Latour, Stephanie, author; Liengola, Innocent, author; Mackaya, Quevain, author; Madidi, Jacob, author; Madzoke, Bola, author; Makoumbou, Calixte, author; Malanda, Guy-Aimé, author; Malonga, Richard, author; Mbani, Olivier, author; Mbendzo, Valentin A., author; Ambassa, Edgar, author; Ekinde, Albert, author; Mihindou, Yves, author; Morgan, Bethan J., author; Motsaba, Prosper, author; Moukala, Gabin, author; Mounguengui, Anselme, author; Mowawa, Brice S., author; Ndzai, Christian, author; Nixon, Stuart, author; Nkumu, Pele, author; Nzolani, Fabian, author; Pintea, Lilian, author; Plumptre, Andrew, author; Rainey, Hugo, author; De Semboli, Bruno Bokoto, author; Serckx, Adeline, author; Stokes, Emma, author; Turkalo, Andrea, author; Vanleeuwe, Hilde, author; Vosper, Ashley, author; Warren, Ymke, author; Public Library of Science, publisher
African forest elephants - taxonomically and functionally unique - are being poached at accelerating rates, but we lack range-wide information on the repercussions. Analysis of the largest survey dataset ever assembled for forest elephants (80 foot-surveys; covering 13,000 km; 91,600 person-days of fieldwork) revealed that population size declined by ca. 62% between 2002-2011, and the taxon lost 30% of its geographical range. The population is now less than 10% of its potential size, occupying less than 25% of its potential range. High human population density, hunting intensity, absence of law enforcement, poor governance, and proximity to expanding infrastructure are the strongest predictors of decline. To save the remaining African forest elephants, illegal poaching for ivory and encroachment into core elephant habitat must be stopped. In addition, the international demand for ivory, which fuels illegal trade, must be dramatically reduced.
Open Access
Effects of 28Si ions, 56Fe ions, and protons on the induction of murine acute myeloid leukemia and hepatocellular carcinoma
(Colorado State University. Libraries, 2014-08) Weil, Michael M., author; Ullrich, Robert L., author; Fallgren, Christina M., author; McCarthy, Maureen, author; Yu, Yongjia, author; Genik, Paula C., author; Ray, F. Andrew, author; Public Library of Science, publisher
Estimates of cancer risks posed to space-flight crews by exposure to high atomic number, high-energy (HZE) ions are subject to considerable uncertainty because epidemiological data do not exist for human populations exposed to similar radiation qualities. We assessed the carcinogenic effects of 300 MeV/n 28Si or 600 MeV/n 56Fe ions in a mouse model for radiation-induced acute myeloid leukemia and hepatocellular carcinoma. C3H/HeNCrl mice were irradiated with 0.1, 0.2, 0.4, or 1 Gy of 300 MeV/n 28Si ions, 600 MeV/n 56Fe ions or 1 or 2 Gy of protons simulating the 1972 solar particle event (1972SPE) at the NASA Space Radiation Laboratory. Additional mice were irradiated with 137Cs gamma rays at doses of 1, 2, or 3 Gy. All groups were followed until they were moribund or reached 800 days of age. We found that 28Si or 56Fe ions do not appear to be substantially more effective than gamma rays for the induction of acute myeloid leukemia. However, 28Si or 56Fe ion irradiated mice had a much higher incidence of hepatocellular carcinoma than gamma ray irradiated or proton irradiated mice. These data demonstrate a clear difference in the effects of these HZE ions on the induction of leukemia compared to solid tumors, suggesting potentially different mechanisms of tumorigenesis. Also seen in this study was an increase in metastatic hepatocellular carcinoma in the 28Si and 56Fe ion irradiated mice compared with those exposed to gamma rays or 1972SPE protons, a finding with important implications for setting radiation exposure limits for space-flight crew members.
Open Access
Effects of recreation on animals revealed as widespread through a global systematic review
(Colorado State University. Libraries, 2016-12-08) Larson, Courtney L., author; Reed, Sarah E., author; Merenlender, Adina M., author; Crooks, Kevin R., author; PLOS ONE, publisher
Outdoor recreation is typically assumed to be compatible with biodiversity conservation and is permitted in most protected areas worldwide. However, increasing numbers of studies are discovering negative effects of recreation on animals. We conducted a systematic review of the scientific literature and analyzed 274 articles on the effects of non-consumptive recreation on animals, across all geographic areas, taxonomic groups, and recreation activities. We quantified trends in publication rates and outlets, identified knowledge gaps, and assessed evidence for effects of recreation. Although publication rates are low and knowledge gaps remain, the evidence was clear with over 93% of reviewed articles documenting at least one effect of recreation on animals, the majority of which (59%) were classified as negative effects. Most articles focused on mammals (42% of articles) or birds (37%), locations in North America (37.7%) or Europe (26.6%), and individual-level responses (49%). Meanwhile, studies of amphibians, reptiles, and fish, locations in South America, Asia, and Africa, and responses at the population and community levels are lacking. Although responses are likely to be species-specific in many cases, some taxonomic groups (e.g., raptors, shorebirds, ungulates, and corals) had greater evidence for an effect of recreation. Counter to public perception, non-motorized activities had more evidence for a negative effect of recreation than motorized activities, with effects observed 1.2 times more frequently. Snow-based activities had more evidence for an effect than other types of recreation, with effects observed 1.3 times more frequently. Protecting biodiversity from potentially harmful effects of recreation is a primary concern for conservation planners and land managers who face increases in park visitation rates; accordingly, there is demand for sciencebased information to help solve these dilemmas.
Open Access
Electrothermal performance of heaters based on laser-induced graphene on aramid fabric
(Colorado State University. Libraries, 2022) Naseri, Iman, author; Ziaee, Morteza, author; Nilsson, Zach N., author; Lustig, Danielle R., author; Yourdkhani, Mostafa, author; American Chemical Society, publisher
Nanostructured heaters based on laser-induced graphene (LIG) are promising for heat generation and temperature control in a variety of applications due to their high efficiency as well as a fast, facile, and highly scalable fabrication process. While recent studies have shown that LIG can be written on a wide range of precursors, the reports on LIG-based heaters are mainly limited to polyimide film substrates. Here, we develop and characterize nanostructured heaters by direct writing of laser-induced graphene on nonuniform and structurally porous aramid woven fabric. The synthesis and writing of graphene on aramid fabric is conducted using a 10.6 μm CO2 laser. The quality of laser-induced graphene and electrical properties of the heater fabric is tuned by controlling the lasing process parameters. Produced heaters exhibit good electrothermal efficiency with steady-state temperatures up to 170 °C when subjected to an input power density of 1.5 W cm–2. In addition, the permeable texture of LIG–aramid fabric heaters allows for easy impregnation with thermosetting resins. We demonstrate the encapsulation of fabric heaters with two different types of thermosetting resins to develop both flexible and stiff composites. A flexible heater is produced by the impregnation of LIG–aramid fabric by silicone rubber. While the flexible composite heater exhibits inferior electrothermal performance compared to neat LIG–aramid fabric, it shows consistent electrothermal performance under various electrical and mechanical loading conditions. A multifunctional fiber-reinforced composite panel with integrated de-icing functionality is also manufactured using one ply of LIG–aramid fabric heater as part of the composite layup. The results of de-icing experiments show excellent de-icing capability, where a 5 mm thick piece of ice is completely melted away within 2 min using an input power of 12.8 W.
Open Access
Evaluating the consistency of subjective activity assessments and their relation to cognition in older adults
(Colorado State University. Libraries, 2021-07-28) Hatt, Cassandra R., author; Brydges, Christopher R., author; Mogle, Jacqueline A., author; Sliwinski, Martin J., author; Bielak, Allison A. M., author; MDPI, publisher
(1) Background: Research examining whether activity engagement is related to cognitive functioning in older adults has been limited to using retrospective reports of activity which may be affected by biases. This study compared two measurements (estimated weekly versus reported daily), and whether these activity assessments were related to cognition in older adults; (2) Methods: Participants from US (n = 199) and Australian (n = 170) samples completed a weekly estimate of activity, followed by 7 consecutive days of daily reporting. Differences between weekly estimates and daily reports were found, such that estimations at the weekly level were lower than self-reported daily information. Multivariate multiple regression was used to determine whether total activity, activity domains and the discrepancy between assessment types (i.e., weekly/daily) predicted cognitive performance across three cognitive domains (fluid, verbal, memory); (3) Results: When activity assessments were totaled, neither predicted cognition; however, when activity was grouped by domain (cognitive, social, physical), different domains predicted different cognitive outcomes. Daily reported cognitive activity significantly predicted verbal performance (β = 1.63, p = 0.005), while weekly estimated social activity predicted memory performance (β = –1.81, p = 0.050). Further, while the magnitude of discrepancy in total activity did not significantly predict cognitive performance, domain specific differences did. Differences in physical activity reported across assessments predicted fluid performance (β = –1.16, p = 0.033); (4) Conclusions: The significant discrepancy between the measurement types shows that it is important to recognize potential biases in responding when conducting activity and cognition research.
Open Access
Evaluation of a novel canine activity monitor for at-home physical activity analysis
(Colorado State University. Libraries, 2015) Yashari, Jonathan M., author; Duncan, Colleen G., author; Duerr, Felix M., author; BioMed Central, publisher
Background: Accelerometers are motion-sensing devices that have been used to assess physical activity in dogs. However, the lack of a user-friendly, inexpensive accelerometer has hindered the widespread use of this objective outcome measure in veterinary research. Recently, a smartphone-based, affordable activity monitor (Whistle) has become available for measurement of at-home physical activity in dogs. The aim of this research was to evaluate this novel accelerometer. Eleven large breed, privately owned dogs wore a collar fitted with both the Whistle device and a previously validated accelerometer-based activity monitor (Actical) for a 24-h time period. Owners were asked to have their dogs resume normal daily activities. Total activity time obtained from the Whistle device in minutes was compared to the total activity count from the Actical device. Activity intensity from the Whistle device was calculated manually from screenshots of the activity bars displayed in the smartphone-application and compared to the activity count recorded by the Actical in the same 3-min time period. Results: A total of 3740 time points were compared. There was a strong correlation between activity intensity of both devices for individual time points (Pearson's correlation coefficient 0.81, p < 0.0001). An even stronger correlation was observed between the total activity data between the two devices (Pearson's correlation coefficient 0.925, p < 0.0001).Conclusions: Activity data provided by the Whistle activity monitor may be used as an objective outcome measurement in dogs. The total activity time provided by the Whistle application offers an inexpensive method for obtaining at-home, canine, real-time physical activity data. Limitations of the Whistle device include the limited battery life, the need for manual derivation of activity intensity data and data transfer, and the requirement of Wi-Fi and Bluetooth availability for data transmission.
Open Access
Evidence of zoonotic leprosy in Pará, Brazilian Amazon, and risks associated with human contact or consumption of armadillos
(Colorado State University. Libraries, 2018-06-28) da Silva, Moises B., author; Portela, Juliana M., author; Li, Wei, author; Jackson, Mary, author; Gonzalez-Juarrero, Mercedes, author; Hidalgo, Andrea Sánchez, author; Belisle, John T., author; Bouth, Raquel C., author; Gobbo, Angélica R., author; Barreto, Josafá G., author; Minervino, Antonio H. H., author; Cole, Stewart T., author; Avanzi, Charlotte, author; Busso, Philippe, author; Frade, Marco A. C., author; Geluk, Annemieke, author; Salgado, Claudio G., author; Spencer, John S., author; Public Library of Science, publisher
Mycobacterium leprae (M. leprae) is a human pathogen and the causative agent for leprosy, a chronic disease characterized by lesions of the skin and peripheral nerve damage. Zoonotic transmission of M. leprae to humans by nine-banded armadillos (Dasypus novemcinctus) has been shown to occur in the southern United States, mainly in Texas, Louisiana, and Florida. Nine-banded armadillos are also common in South America, and residents living in some areas in Brazil hunt and kill armadillos as a dietary source of protein. This study examines the extent of M. leprae infection in wild armadillos and whether these New World mammals may be a natural reservoir for leprosy transmission in Brazil, similar to the situation in the southern states of the U.S. The presence of the M. leprae-specific repetitive sequence RLEP was detected by PCR amplification in purified DNA extracted from armadillo spleen and liver tissue samples. A positive RLEP signal was confirmed in 62% of the armadillos (10/16), indicating high rates of infection with M. leprae. Immunohistochemistry of sections of infected armadillo spleens revealed mycobacterial DNA and cell wall constituents in situ detected by SYBR Gold and auramine/rhodamine staining techniques, respectively. The M. leprae-specific antigen, phenolic glycolipid I (PGL-I) was detected in spleen sections using a rabbit polyclonal antibody specific for PGL-I. Anti-PGL-I titers were assessed by ELISA in sera from 146 inhabitants of Belterra, a hyperendemic city located in western Pará state in Brazil. A positive anti-PGL-I titer is a known biomarker for M. leprae infection in both humans and armadillos. Individuals who consumed armadillo meat most frequently (more than once per month) showed a significantly higher anti-PGL-I titer than those who did not eat or ate less frequently than once per month. Armadillos infected with M. leprae represent a potential environmental reservoir. Consequently, people who hunt, kill, or process or eat armadillo meat are at a higher risk for infection with M. leprae from these animals.