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Browsing Theses and Dissertations by Subject "acoustic contrast factor"

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    ItemOpen Access
    Development of a continuous flow ultrasonic harvesting system for microalgae
    (Colorado State University. Libraries, 2014) Hincapié Gómez, Esteban, author; Marchese, Anthony J., advisor; Willson, Bryan D., committee member; Dasi, Lakshmi Prasad, committee member; Peers, Graham, committee member
    Microalgae have vast potential as a sustainable source of biofuel. However, numerous technoeconomic analyses have indicated that microalgae harvesting represents a critical bottleneck in the microalgae value chain in terms of energy requirements, capital cost and operating cost. This dissertation presents an approach that uses a combination of acoustophoretic, fluid mechanical, and gravitational forces toward the development of a continuous flow microalgae harvesting system. Ultrasonic Standing Waves have been widely reported in the literature as an approach to manipulate particles in a fluid, a phenomena known as acoustophoresis. These waves exert an acoustic force that agglomerate the cells in the wave nodes or antinodes and the force is directly proportional to the cell acoustic contrast factor. Ultrasonic microalgae harvesting is a promising low cost and low energy approach. However, a better understanding of the acoustic properties of microalgae is essential for the development of this technology. Accordingly, a major component of this work focused on accurately quantifying the acoustic contrast factor of microalgae cells of Nannochloropsis oculata, Nannochloropsis gaditana, Phaeodactylum tricornutum and Chlamydomonas reinhardtii by measuring the average cell density and speed of sound using a vibrating tube densitometer. The results indicate a linear correlation of density and speed of sound as a function of cell concentration. Using this correlation, non-scattering volume average relationships were used to compute density and speed of sound for the average algal cell. The acoustic contrast factor was estimated to be between 0.04 - 0.06 for microalgae cells in their corresponding growth media. Second, particle tracking velocimetry was used to determine the magnitude of the acoustophoretic force. In these studies, in addition to microalgae cells, polyamide seeding particles were used as a surrogate. The results obtained conclude that the maximum acoustophoretic forces are approximately 5 pN for Chlamydomonas reinhardtii cells and the results also show that there is change in the acoustic contrast factor from positive to negative with lipid accumulation. This dissertation also presents a novel device for the acoustic harvesting of microalgae. The design is based on using the acoustophoretic force, acoustic transparent materials and inclined settling (Boycott effect). A filtration efficiency of 70% ± 5% and a concentration factor of 11.6 ± 2.2 were achieved at a flow rate of 25 mL • min-1 and an energy consumption of 3.6 ± 0.9 kWh • m-3. The effects of the applied power, flow rate, inlet cell concentration and inclination were explored. It was found that the filtration efficiency of the device is proportional to the power applied. However, the filtration efficiency experienced a plateau at a 100 W • L-1 of power density applied. The filtration efficiency also increased with increasing inlet cell concentration and was inversely proportional to the throughput of the device as measured flow rate. It was also found that the optimum settling angle for maximum concentration factor occurred at an angle of 50° ± 5°. At these optimum conditions, the device had higher filtration efficiency in comparison to other similar devices reported in the previous literature.
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    ItemOpen Access
    Implications of cell composition and size on the performance of microalgae ultrasonic harvesting
    (Colorado State University. Libraries, 2018) Aligata, Alyssa Jean, author; Marchese, Anthony, advisor; Quinn, Jason, advisor; Peebles, Christie, committee member
    Substantial economic challenges exist across the value chain for microalgae-based biofuels and bioproducts. Acoustic harvesting could dramatically reduce harvesting costs and directly address current energy barriers to separating algae from growth media. This technology utilizes ultrasonic standing waves to create an acoustic radiation force that, due to differences in the acoustic properties of the cells and media, causes the microalgae cells to agglomerate and settle out of the solution. The magnitude of the acoustic radiation force is directly related to the cell radius and acoustic contrast factor (ACF), the latter of which is a function of the density and compressibility of the cell. These properties can vary widely depending on the algae species, cultivation conditions, and growth stage—all of which affect the composition of the microalgae cells (e.g., lipid, carbohydrate, protein content). In this work, two methods were used to determine the ACF of microalgal cells: 1) a property measurement approach and 2) a particle tracking approach. The first method involved experimentally measuring the size distribution, density and compressibility of the cells and calculating the ACF. The second method utilized particle tracking velocimetry and a COMSOL Multiphysics model to estimate the ACF. The ACF was characterized, using both techniques, for three species—Chlamydomonas reinhardtii, Nannochloropsis salina, and Tetraselmis chuii—as a function of dynamic cellular composition over a 2-week growth period. For C. reinhardtii the lipid content increased from 26% ± 1% to 40% ± 1% from day 3 to 9, which resulted in a 43% decrease in ACF (0.056 ± 0.003 to 0.032 ± 0.001). For N. salina the lipid content increased from 25% ± 1% to 33% ± 1% from day 3 to 10, which also resulted in a 43% decrease in ACF (0.040 ± 0.002 to 0.023 ± 0.001). For T. chuii the lipid content remained relatively stable (~10%) throughout the growth period so the ACF (~0.3) did not change significantly. ACF decreases as lipid content increases because lipids have a negative ACF in growth media, whereas carbohydrates and proteins have a positive ACF. However, cell size can have a greater impact on an algal strains' responsiveness to acoustic harvesting because the net force is proportional to Φa2. Furthermore, acoustic harvesting works best for large diameter cells, provided that those cells have a nonzero ACF. T. chuii had the largest cell diameter of approximately 12 µm, while C. reinhardtii and N. salina had cell diameters of 8.5 µm and 4.3 µm, respectively. The Φa2 values for T. chuii were approximately 50× higher than the values for N. salina, which is largely due to T. chuii cells having a diameter that is 3× the diameter of N. salina cells. Composition also contributed to the higher Φa2 values for T. chuii since these cells were composed of mostly carbohydrates and had an ACF that was an order of magnitude higher than the ACF of N. salina. This research shows that acoustic harvesting has the potential to positively impact the algal biofuels value chain through the reduction of energy required for harvesting.