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Browsing by Author "Geornaras, Gina, committee member"

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    Efficacy and impact of sanitizers in controlling pathogenic bacteria on irrigation water
    (Colorado State University. Libraries, 2024) Santillan Oleas, Valeria Paola, author; Gutierrez-Rodriguez, Eduardo, advisor; Geornaras, Gina, committee member; Uchanski, Mark, committee member; Ma, Jianbing, committee member
    Irrigation water quality is crucial for fresh produce. However, the absence of practical microbial water standards and EPA-registered sanitizers poses challenges for growers, impacting food security and increasing the risk of foodborne outbreaks. This study compared the effectiveness of commercial sanitizers (sodium hypochlorite - Chlo, peroxyacetic acid - PAA) and novel chemistries (sodium acid sulfate - SAS, potassium acid sulfate - KAS) at different concentrations for achieving a 3-log reduction of E. coli O157:H7 (EHEC), Salmonella (SM), or Listeria monocytogenes (LM). Additionally, the study examined their corrosive effects on aluminum and stainless steel, as well as their impact on plant and soil chemical and physical properties. Sanitizer evaluations were conducted under varying turbidities (0, 50, 100, 150 NTU), contact times (CT: 3 or 5 minutes), and bacterial concentrations of EHEC, SM, or LM at 6 and 3 log CFU/mL per EPA 2020 guidelines. Treatments that achieved a 3-log reduction of the selected organisms within a 5-minute contact time, with no viable cells after enrichment, were considered the most effective and exceeded EPA requirements. These treatments were then evaluated for their corrosion capacity on stainless steel (SS304, SS316) and aluminum (AL3004) coupons, as well as their effects on soil and plant health (romaine lettuce) under greenhouse conditions. Chlo at 60 ppm, SAS at 0.60%, and PAA at 30 ppm achieved a 3-log reduction, with no viable cells after enrichment, for all strains after a 5-minute contact time (P<0.05). The corrosion capacity for the most effective treatments was tested at 14-day intervals at 23°C and 73°C for stainless steel (SS304, SS316) and aluminum (AL3004) coupons submerged in each sanitizer solution. There was no significant difference in corrosion between SS304 and SS316 (P<0.05). AL3004 showed greater corrosion than SS, with the highest rate observed at 0.6% SAS (4.52E-02 cm/year). Greenhouse-grown romaine lettuce was spray-inoculated with a bacterial cocktail of LM/EHEC at 5 log CFU/mL. Bacterial recovery was performed at 0-, 4-, 8-, and 12-days post-inoculation using selective-differential media. Soil samples were collected from treated and non-treated samples to assess soil chemical and physical properties. Bacterial inactivation had a negative correlation with water turbidity and a positive correlation with contact time and sanitizer concentration (P<0.05). In the greenhouse trial PAA, Chlorine and SAS exhibited a higher bacterial reduction compared to a water-control, with marginal differences in reduction between sanitizers (P<0.05). The highest reduction was achieved by PAA (0.98 log CFU/gram-lettuce) reduction compared to the water control. SAS exhibited phytotoxicity in the lettuce leaves in the second greenhouse trial. All sanitizers caused marginal changes in the overall properties of soil physical and chemical properties, with sodium and electrical conductivity being the main factors affected by the constant application of each sanitizer.
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    In vivo and in vitro attributes of copper, zinc, and manganese sources in beef cattle nutrition
    (Colorado State University. Libraries, 2023) Guimaraes, Octavio, author; Engle, Terry T., advisor; Wagner, John, committee member; Geornaras, Gina, committee member; Nair, Mahesh, committee member; Holt, Tim, committee member
    Trace minerals are not just vital components in the diets of domestic livestock species; they are also essential for ensuring optimal performance, health, reproduction, and a robust immune system. In today's volatile markets, producers and nutritionists face the challenge of meeting predefined goals and targets. They must employ strategies that can maximize production and efficiency. The intricate mechanisms governing the digestion, absorption, and storage of copper (Cu), zinc (Zn), and manganese (Mn) are influenced by various factors, including diet type, breed, life stage, among many others. Research has uncovered intriguing opportunities to enhance the utilization of trace minerals (TM) in ruminant nutrition. To further our comprehension of the digestion, absorption, and retention of modern trace mineral forms, this study conducted 3 experiments aimed at investigating the in vivo and in vitro characteristics of hydroxy trace minerals (HTM), organic chelated trace minerals (CTM), and sulfate trace minerals (STM) forms of Cu, Zn, and Mn. This dissertation is the continuation of a Master Thesis submitted by Guimaraes et al., 2021. In the current series of experiments the influence of TM source on digestibility and ruminal solubility was investigated. The objectives of experiment 1 were to determine the effects of TM source on 1) nutrient digestibility and ruminal fermentation, 2) concentrations of soluble Cu, Zn, and Mn in the rumen following a pulse dose of TM, and 3) Cu, Zn, and Mn binding strength on ruminal digesta using dialysis against a chelating agent in steers fed a diet formulated to meet the requirements of a high producing dairy cow. Twelve Angus steers fitted with ruminal cannulae were adapted to a diet balanced with nutrient concentrations similar to a diet for a high producing lactating dairy cow for 21 d. Steers were then randomly assigned to dietary treatments consisting of 10 mg Cu, 40 mg Mn, and 60 mg Zn/kg DM from either sulfate (STM), hydroxychloride (HTM) or complexed trace minerals (CTM). The experimental design did not include a negative control (no supplemental Cu, Mn, or Zn) because the basal diet did not meet the NRC (Nutrient Requirement for Beef Cattle) requirement for Cu and Zn. Copper, Mn, and Zn are also generally supplemented to lactating dairy cow diets at concentrations approximating those supplied in the present study. Following a 14-d adaptation period, total fecal output was collected for 5-d. Following the fecal collection period, rumen fluid was collected for VFA parameters. On the following day, the same diet was provided for 14 d, without supplemental Cu, Zn, and Mn. This period served as a wash-out period. A pulse dose of 100, 400, and 600 mg of Cu, Zn, Mn, respectively, from either STM, HTM, or CTM, was administered via ruminal cannulae to the steers on day 15. Over a 24-h period ruminal samples were obtained every 2-h. Following centrifugation, the supernatant was analyzed for Cu, Mn, and Zn. Ruminal solid digesta samples from times 0, 12, and 24 hours after bolus dosing were exposed to dialysis against Tris-EDTA. The digestibility of NDF and ADF were lesser in STM vs. HTM and vs. CTM supplemented steers. Steers receiving HTM and CTM had greater total VFA concentrations than STM, and molar proportions of individual VFA were not affected by treatment. Ruminal soluble Cu and Zn concentrations were greater post dosing in STM and CTM supplemented steers at 2, 4, and 6 h for Cu and 4, 6, 8, 10 and 12 h for Zn when compared to HTM supplemented steers. The release of Cu and Zn from ruminal solid digesta following dialysis against Tris-EDTA at 12 and 24 h post-dosing was greater for steers receiving HTM compared to those receiving STM or CTM. Results indicate trace mineral source impacts: 1) how tightly bound Cu and Zn are to ruminal solid digesta; 2) fiber digestion; 3) and ruminal total VFA concentrations. The objective of experiment 2 was to evaluate the effects of sources of STM and HTM fed at the same levels on 1) nutrient digestibility and fermentation characteristics, 2) ruminal solubility of Cu, Zn, and Mn following a pulse dose of trace mineral I, and 3) binding strength of Cu, Zn, and Mn on ruminal solid digesta upon dialysis against a chelating agent. Minor dietary changes can have a direct impact on ruminal pH, temperature, microbial population, and redox potential, which will influence fermentation, and ultimately affect nutrient digestibility. These changes can influence or be influenced by the solubility of certain TM in the rumen. Rumen soluble TMs can alter nutrient digestibility, fermentation, and increase chances of antagonistic interaction. Twelve ruminally cannulated Angus steers (BW 587.6 ± 23.1 kg) were adapted to a diet balanced to meet requirements of finishing steers for 21 d. Steers were then randomly assigned to dietary treatments consisting of Cu, Mn, and Zn supplemented at 18, 40, and 90 mg/kg DM, respectively, from STM or HTM sources (n = 6 steers/treatment; experimental unit = steer). Following the 14 days treatment adaptation period total fecal output was collected for 5 d. Digestibility of DM, CP, Starch, NDF, and ADF was not affected (P > 0.15) by TM source. On d 6, rumen fluid was collected at 0, 2, and 4 h post feeding for VFA analysis. There were no treatment x time interactions for any VFA measured. However, HTM steers had greater (P < 0.05) ruminal molar proportions of propionate and tended (P < 0.07) to have greater molar proportions of valerate compared to STM steers. Steers fed STM had greater (P < 0.05) molar proportions of butyrate compared to HTM steers. Steers were then fed the same high concentrate diet without supplemental Cu, Zn, or Mn for 14 d. On d 15 steers received a pulse dose (via rumen cannulae) from either STM or HTM. Ruminal samples were obtained at 2 h intervals starting at -4 and ending at 24 h relative to dosing. There was a treatment x time interaction (P < 0.05) for ruminal soluble Cu concentrations. Ruminal soluble mineral concentrations were greater (P < 0.05) for Cu at 4, 6, 8, and 16 h post dosing in STM compared to HTM supplemented steers. There was no treatment x time interaction for ruminal soluble Zn or Mn concentrations. The release of Cu, Zn, and Mn from ruminal solid digesta following dialysis against Tris-EDTA at 12 and 24 h post-dosing was greater (P < 0.01) for steers receiving HTM compared to those receiving STM. Results of this experiment indicate that trace mineral source affects how tightly bound Cu, Zn, and Mn are to ruminal solid digesta, and that ruminal solubility of Cu differs between STM and HTM sources and may impact rumen fermentation characteristics. Experiment 3: This study analyzed samples from experiments investigating TM source effects on nutrient digestibility, short-chain fatty acid production, Cu, Zn, and Mn ruminal solubility, and binding strength in rumen insoluble digesta. Twelve Angus steers (5+ years old) participated in three studies: Study 1 (Guimaraes et al., 2020, 2021; medium quality forage diet), Study 2 (Guimaraes et al., 2022; dairy type diet), and Study 3 (unpublished, Chapter 3 of this dissertation; high concentrate diet). Due to impacts on digestibility, fermentation, mineral solubility, and binding in all studies, microbiome analysis was conducted to assess potential bacterial and protozoa population shifts from all studies. In Study 1 (medium quality forage diet), Papillibacter was the predominant species in the rumen. Rumen samples showed a significant time point impact on Shannon's PD (P < 0.05). Beta diversity analysis using unweighted UniFrac revealed notable differences in community diversity between rumen and fecal samples (q < 0.001). Rumen microbiota clustered at multiple time points. No beta diversity differences between STM and HTM were observed at baseline (Time A, q = 0.23), but differences emerged after adaptation (Time C, q < 0.03) and during total collection (Time F, q < 0.01). ANCOM analysis revealed higher abundance of Papillibacter, Ruminoccoccaceae, and Prevotellaceae in rumen samples, regardless of TM source. No significant differences were found in fecal samples. ANCOM analysis did not detect measurable differential abundance between trace mineral sources in fecal or rumen samples following Alpha and Beta diversity results. In Study 2 (dairy diet), Moraxellaceae was the most prevalent species within the rumen. Rumen samples showed a significant time point effect on Shannon's PD (P < 0.001), with noteworthy differences between several time points. However, TM source had no impact on Shannon's PD (P = 0.15). Fecal samples showed no differences in time points or TM sources. Beta diversity analysis using unweighted UniFrac revealed a significant distinction between microbial community diversity in rumen and fecal samples (q < 0.001). Rumen microbiota clustered differently at various time points. Trace mineral source did not affect Beta diversity, but there was a tendency toward greater diversity with HTM supplementation (q = 0.07). ANCOM analysis found higher abundance of ASVs Moraxellaceae, Planococcaceae, Ruminoccoccaceae, and Prevotellaceae in the rumen environment, regardless of TM source. No significant differential abundance was detected between TM sources in rumen samples, consistent with Shannon's PD diversity analysis. In Study 3 (high concentrate diet), Moraxellaceae and Planococcaceae were the most abundant in rumen samples. Rumen samples showed a significant impact of collection time point on Shannon’s PD (P < 0.001), with significant differences between several time points. However, TM source had no effect on Shannon’s Diversity (P = 0.19). Interestingly, Shannon’s Diversity in feces showed significant differences between TM sources (P < 0.03), with STM having lesser diversity than HTM. There was no effect of time point in fecal samples (P = 0.38). Beta diversity analysis using unweighted UniFrac revealed clear differences between microbial diversity in rumen and fecal samples (q < 0.001). ANCOM analysis found higher abundance of ASVs Moraxellaceae, Prevotellaceae, Planococcaceae, and Ruminoccoccaceae in the rumen environment, regardless of TM source. In conclusion, this study provides valuable insights into the complex interplay between TM sources, the rumen microbiome, and various aspects of rumen health and function. While TM sources did not significantly alter the rumen microbiome composition, time points demonstrated substantial effects on microbial communities. These findings contribute to our understanding of how dietary factors can influence rumen ecology and function, with potential implications for livestock nutrition and management strategies. In conclusion, while experiment 1 showed that HTM and CTM sources positively impacted fiber digestibility and ruminal VFA concentrations compared to STM, experiment 2 indicated that the diet type could influence these effects. However, ruminal soluble mineral concentrations remained greater in STM-supplemented steers. Experiment 3 further highlighted the differences in rumen and fecal microbiota diversity between TM sources. These findings underscore the importance of selecting the appropriate TM source based on specific dietary and production requirements. The interactions between TMs, diet type, and the rumen microbiome are complex and require further investigation to optimize ruminant nutrition and overall well-being. This research builds upon previous studies and contributes valuable insights into the utilization of TMs in ruminant nutrition, paving the way for more informed decision-making in livestock production practices.