Title: Dataset associated with "mRNA localization is linked to translation regulation in the
Caenorhabditis elegans germ lineage"

Abstract: Caenorhabditis elegans early embryos generate cell-specific
transcriptomes despite lacking active transcription. This presents an
opportunity to study mechanisms of post-transcriptional regulatory
control. In seeking the mechanisms behind this patterning, we discovered
that some cell-specific mRNAs accumulate non-homogenously within cells,
localizing to membranes, P granules (associated with progenitor germ
cells in the P lineage), and P-bodies (associated with RNA processing).
Transcripts differed in their dependence on 3'UTRs and RNA Binding
Proteins, suggesting diverse regulatory mechanisms. Notably, we found
strong but imperfect correlations between low translational status and P
granule localization within the progenitor germ lineage. By uncoupling
these, we untangled a long-standing question: Are mRNAs directed to P
granules for translational repression or do they accumulate there as a
downstream step? We found translational repression preceded P granule
localization and could occur independent of it. Further, disruption of
translation was sufficient to send homogenously distributed mRNAs to P
granules. Overall, we show transcripts important for germline
development are directed to P granules by translational repression, and
this, in turn, directs their accumulation in the progenitor germ lineage
where their repression can ultimately be relieved.

Authors: Dylan M. Parker, Lindsay P. Winkenbach, Sam Boyson, Matthew N.
Saxton, Camryn Daidone, Zainab A. Al-Mazaydeh, Marc T. Nishimura,
Florian Mueller, and Erin Osborne Nishimura

Contact: erin.nishimura@colostate.edu

License info: This is an Open Access article distributed under the terms
of the Creative Commons Attribution License
(https://creativecommons.org/licenses/by/4.0), which permits
unrestricted use, distribution and reproduction in any medium provided
that the original work is properly attributed.

Publication citation: 
Parker, D. M., Winkenbach, L. P., Boyson, S., Saxton, M. N., Daidone, C., Al-Mazaydeh1, Z. A., Nishimura, M. T., Mueller, F., & Nishimura, E. O. (2020). mRNA localization is linked to translation regulation in the Caenorhabditis elegans germ lineage. Development. http://dx.doi.org/10.1242/dev.186817

PMID: 32541012

Data citation: 
Parker, D. M., Winkenbach, L. P., Boyson, S., Saxton, M. N., Daidone, C., Al-Mazaydeh1, Z. A., Nishimura, M. T., Mueller, F., & Nishimura, E. O. (2020). Dataset associated with "mRNA localization is linked to translation regulation in the Caenorhabditis elegans germ lineage". Colorado State University. Libraries. http://dx.doi.org/10.25675/10217/201623

Format of data files: .tif and .txt files are contained in this repository

Data were collected at Colorado State University, Fort Collins, CO 80524

Data were collected between 2015 and 2020.

File information: Files are compressed using .tar.bz2 and can be extracted using the command line command: tar -xvjf "file_name".tar.bz2
Each .tar.bz2 file contains either all or part of the associated raw data for any particular figure, represented by the name of the file.
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Methods: All images were acquired on a Photometrics Cool Snap HQ2 camera using a DeltaVision Elite inverted microscope (GE Healthcare), with an Olympus PLAN APO 60X (1.42 NA, PLAPON60XOSC2) objective, an Insight SSI 7-Color Solid State Light Engine, and SoftWorx software (Applied Precision) using 0.2 um z-stacks.